LIN28B Antikörper (Lin-28 Homolog B (C. Elegans)) (AA 242-246)

Details for Product anti-LIN28B Antibody No. ABIN6267754
Target
Bindungsspezifität
AA 242-246
12
10
7
5
4
3
3
2
1
1
1
1
1
Reaktivität
Human
61
20
14
1
1
1
1
1
Wirt
Kaninchen
61
1
Klonalität
Polyklonal
Konjugat
Dieser LIN28B Antikörper ist unkonjugiert
5
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
44
30
30
28
22
10
10
6
4
2
1
Optionen
Immunogen Peptide sequence around aa.242~246( P-S-V-Q-K) derived from Human LIN28B
Isotyp IgG
Spezifität The antibody detects endogenous levels of total LIN28B protein.
Kreuzreaktivität Human
Reinigung The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Target
Andere Bezeichnung LIN28B (LIN28B Antibody Abstract)
Hintergrund

Description: Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463). Does not act on pri-miR21 (PubMed:22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed:22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Gene: LIN28B

Molekulargewicht 21 32kDa
Gen-ID 389421
UniProt Q6ZN17
Applikations-hinweise WB 1:500-1:1000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Bilder
Image no. 1 for anti-Lin-28 Homolog B (C. Elegans) (LIN28B) (AA 242-246) antibody (ABIN6267754) Western blot analysis of extracts from Hela and 293 cells using LIN28B Antibody