CDK4 Antikörper (Cyclin-Dependent Kinase 4) (AA 295-299)

Details for Product anti-CDK4 Antibody No. ABIN6267732
Target
Bindungsspezifität
AA 295-299
28
16
15
10
10
9
8
7
7
5
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
Reaktivität
Human
146
83
65
13
6
5
5
4
3
1
1
1
Wirt
Kaninchen
118
48
4
2
1
Klonalität
Polyklonal
Konjugat
Dieser CDK4 Antikörper ist unkonjugiert
15
11
8
5
5
4
3
3
3
3
3
3
2
2
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
131
70
38
29
29
24
13
13
12
4
4
4
4
2
2
2
1
1
Optionen
Immunogen Peptide sequence around aa.295~299(L-H-K-D-E)derived from Human CDK4.
Isotyp IgG
Spezifität The antibody detects endogenous levels of total CDK4 protein.
Kreuzreaktivität Human
Reinigung The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Target
Andere Bezeichnung CDK4 (CDK4 Antibody Abstract)
Hintergrund

Description: Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Gene: CDK4

Molekulargewicht 34kDa
Gen-ID 1019
UniProt P11802
Pathways Zellzyklus, Mitotic G1-G1/S Phases, Regulation of Cell Size
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Bilder
Image no. 1 for anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 295-299) antibody (ABIN6267732) Western blot analysis of extracts from HUVEC cells using CDK4 Antibody
Produkt verwendet in: Wu, Herman, Brock, Wu, Mao, Yan, Nie, Liang, Zhan, Li, Guo: "Silencing DACH1 promotes esophageal cancer growth by inhibiting TGF-β signaling." in: PLoS ONE, Vol. 9, Issue 4, pp. e95509, 2015 (PubMed).