PKM1/2 (AA 36-40) Antikörper

Details zu Produkt Nr. ABIN6267730
Target
Bindungsspezifität
AA 36-40
1
Reaktivität
Human
9
Wirt
Kaninchen
9
Klonalität
Polyklonal
Konjugat
Unkonjugiert
1
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
4
1
1
1
Optionen
Immunogen Peptide sequence around aa. 36~40(D-S-P-P-I) derived from Human PKM1/2.
Isotyp IgG
Spezifität The antibody detects endogenous levels of total PKM1/2 protein.
Kreuzreaktivität Human
Reinigung The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Target
Hintergrund

Description: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Gene: PKM

Molekulargewicht 60kDa
Gen-ID 5315
UniProt P14618
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Bilder
Image no. 1 for anti-PKM1/2 (AA 36-40) antibody (ABIN6267730) Western blot analysis of extracts from HepG2 and 293 cells using PKM1/2