Nanog Homeobox (NANOG) (AA 137-141) Antikörper

Details zu Produkt Nr. ABIN6267726
Target
Bindungsspezifität
AA 137-141
16
15
14
12
11
11
10
8
8
8
7
5
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
186
63
42
4
4
4
4
2
2
2
1
1
1
Wirt
Kaninchen
163
37
10
2
Klonalität
Polyklonal
Konjugat
Unkonjugiert
15
15
13
11
10
10
4
4
4
4
4
4
2
2
2
2
2
Applikation
ELISA, Western Blotting (WB)
145
112
64
52
26
26
22
20
17
9
6
3
2
2
1
1
1
1
Optionen
Immunogen Peptide sequence around aa.137~141 (K-Q-V-K-T) derived from Nano g
Isotyp IgG
Spezifität The antibody detects endogenous level of total Nanog protein.
Kreuzreaktivität Human, Maus
Reinigung The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Target
Andere Bezeichnung Nanog (NANOG Antibody Abstract)
Hintergrund

Description: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Gene: NANOG

Molekulargewicht 42kDa
Gen-ID 79923
UniProt Q9H9S0
Pathways Stem Cell Maintenance
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Bilder
Image no. 1 for anti-Nanog Homeobox (NANOG) (AA 137-141) antibody (ABIN6267726) Western blot analysis of extracts from human Umbilical cord mesenchymal stem cell usi...