MAPK14 Antikörper (Mitogen-Activated Protein Kinase 14) (pThr180)

Details for Product anti-MAPK14 Antibody No. ABIN6267706
Target
  • CSBP
  • CSBP1
  • CSBP2
  • CSPB1
  • EXIP
  • Mxi2
  • PRKM14
  • PRKM15
  • RK
  • SAPK2A
  • p38
  • p38ALPHA
  • CRK1
  • Csbp1
  • Csbp2
  • Exip
  • Hog
  • Prkm14
  • Prkm15
  • Sapk2A
  • p38Hog
  • p38alpha
  • p38b
  • zp38b
  • MAPK14
  • 186F5S
  • BG:DS00797.3
  • CG7393
  • D-p38
  • D-p38 MAPK
  • D-p38b
  • Dm p38b
  • Dmel\\CG7393
  • Dmp38b
  • Dp38
  • Dp38b
  • ESTS:186F5S
  • Mpk34C
  • anon-sts23
  • dp38b
  • p38 MAPK
  • p38 beta
  • p38B
  • p38Kb
  • p38beta
  • Crk1
  • p38-alpha
  • p38MAPK
  • p38a
  • csbp
  • mapk14a
  • mxi2
  • sapk2
  • sapk2a
  • AP22.98
  • AP22_98
  • ATMPK14
  • mitogen-activated protein kinase 14
  • SAPK2a
  • MAP kinase 14A
  • MAP kinase p38a
  • MAPK 14A
  • fk28c03
  • hm:zeh1243
  • wu:fk28c03
  • zp38a
  • P38C-CRK
  • mitogen-activated protein kinase 14
  • mitogen activated protein kinase 14
  • mitogen-activated protein kinase 14b
  • p38b MAP kinase
  • mitogen-activated protein kinase 14 S homeolog
  • mitogen-activated protein kinase 14a
  • CRK proto-oncogene, adaptor protein
  • MAPK14
  • Mapk14
  • mapk14b
  • p38b
  • mapk14.S
  • MPK14
  • mapk14a
  • CRK
Bindungsspezifität
pThr180, pTyr182
48
40
19
15
15
15
13
12
12
11
8
8
8
7
6
6
5
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
334
232
209
56
42
40
39
35
34
34
28
28
17
15
6
3
3
2
1
1
1
1
Wirt
Kaninchen
266
69
2
2
1
Klonalität
Polyklonal
Konjugat
Dieser MAPK14 Antikörper ist unkonjugiert
22
14
11
9
9
8
8
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Applikation
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
270
150
135
90
67
42
28
28
26
24
15
6
6
5
5
2
2
1
1
1
1
1
1
Optionen
Immunogen A synthesized peptide derived from human p38 MAPK around the phosphorylation site of Thr180/Tyr182 .
Isotyp IgG
Spezifität Phospho-p38 MAPK (Thr180/Tyr182) Antibody detects endogenous levels of p38 MAPK only when phosphorylated at Thr180 and Tyr182 .
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung p38 MAPK (MAPK14 Antibody Abstract)
Hintergrund

Description: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. For example, CFLAR is an inhibitor of TNF-induced apoptosis whose proteasome-mediated degradation is regulated by p38 MAPK phosphorylation. In a similar way, MAPK14 phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Another function of MAPK14 is to regulate the endocytosis of membrane receptors by different mechanisms that impinge on the small GTPase RAB5A. In addition, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14-mediated phosphorylation of EGFR itself as well as of RAB5A effectors. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17. Such phosphorylation is required for ADAM17-mediated ectodomain shedding of TGF-alpha family ligands, which results in the activation of EGFR signaling and cell proliferation. Another p38 MAPK substrate is FGFR1. FGFR1 can be translocated from the extracellular space into the cytosol and nucleus of target cells, and regulates processes such as rRNA synthesis and cell growth. FGFR1 translocation requires p38 MAPK activation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, DDIT3, TP53/p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. This phosphorylation enhances the accessibility of the cryptic NF-kappa-B-binding sites marking promoters for increased NF-kappa-B recruitment. Phosphorylates CDC25B and CDC25C which is required for binding to 14-3-3 proteins and leads to initiation of a G2 delay after ultraviolet radiation. Phosphorylates TIAR following DNA damage, releasing TIAR from GADD45A mRNA and preventing mRNA degradation. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation. Protein O-Glc-N-acylation catalyzed by the OGT is regulated by MAPK14, and, although OGT does not seem to be phosphorylated by MAPK14, their interaction increases upon MAPK14 activation induced by glucose deprivation. This interaction may regulate OGT activity by recruiting it to specific targets such as neurofilament H, stimulating its O-Glc-N-acylation. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Also plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis. Phosphorylates S100A9 at 'Thr-113'.

Gene: MAPK14

Molekulargewicht 43 kDa
Gen-ID 1432
UniProt Q16539
Pathways MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagie, Thromboxane A2 Receptor Signaling
Applikations-hinweise WB 1:500-1:2000 IHC 1:50-1:200 IF 1:200 IP
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Bilder
Image no. 1 for anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180), (pTyr182) antibody (ABIN6267706) ABIN6267707 at 1/200 staining human kidney tissue sections by IHC-P.
Image no. 2 for anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180), (pTyr182) antibody (ABIN6267706) ABIN6267707 staining 293T treated with UV 30min by IF/ICC. The sample were fixed with...
Image no. 3 for anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180), (pTyr182) antibody (ABIN6267706) Western blot analysis of p38 MAPK phosphorylation expression in Jurkat whole cell lys...
Produkt verwendet in: Yang, Wu, Jia, Zhao, Hou, Liu, Wang, Chen, Yang, Lei, Wang: "The mechanically activated p38/MMP-2 signaling pathway promotes bone marrow mesenchymal stem cell migration in rats." in: Archives of oral biology, Vol. 76, pp. 55-60, 2018 (PubMed).

Geng, Yang, Wang, Zhang, Hu, Gu, Li: "Trimethylamine N-oxide promotes atherosclerosis via CD36-dependent MAPK/JNK pathway." in: Biomedicine & pharmacotherapy, Vol. 97, pp. 941-947, 2018 (PubMed).

Huang, Chen, Zhang, Li, Chen, Yang, Liang, Zhu, Xu: "Neuroprotective Effect of Curcumin Against Cerebral Ischemia-Reperfusion Via Mediating Autophagy and Inflammation." in: Journal of molecular neuroscience : MN, Vol. 64, Issue 1, pp. 129-139, 2018 (PubMed).

Cao, Liu, Huang, Wang, Jiao, Huo: "SUMO2 modification of Aurora B and its impact on follicular development and atresia in the mouse ovary." in: International journal of molecular medicine, Vol. 41, Issue 6, pp. 3115-3126, 2018 (PubMed).

Xing, Yang, Wang, Dong, Feng, Zhang: "Electroacupuncture Alleviated Neuronal Apoptosis Following Ischemic Stroke in Rats via Midkine and ERK/JNK/p38 Signaling Pathway." in: Journal of molecular neuroscience : MN, Vol. 66, Issue 1, pp. 26-36, 2018 (PubMed).

Liu, Zheng, Zhang, Wang, Yang, Bai, Dai: "Fucoxanthin Activates Apoptosis via Inhibition of PI3K/Akt/mTOR Pathway and Suppresses Invasion and Migration by Restriction of p38-MMP-2/9 Pathway in Human Glioblastoma Cells." in: Neurochemical research, Vol. 41, Issue 10, pp. 2728-2751, 2017 (PubMed).

Wang, Liu, Wang, Fan, Wang, Wang: "Oxymatrine inhibits the migration of human colorectal carcinoma RKO cells via inhibition of PAI-1 and the TGF-β1/Smad signaling pathway." in: Oncology reports, Vol. 37, Issue 2, pp. 747-753, 2017 (PubMed).