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RAG2 Antikörper (N-Term)

RAG2 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN6264622
  • Target Alle RAG2 Antikörper anzeigen
    RAG2 (Recombination Activating Gene 2 (RAG2))
    Bindungsspezifität
    • 15
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reaktivität
    • 45
    • 13
    • 12
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 39
    • 7
    Kaninchen
    Klonalität
    • 41
    • 5
    Polyklonal
    Konjugat
    • 22
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser RAG2 Antikörper ist unkonjugiert
    Applikation
    • 20
    • 13
    • 13
    • 11
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Spezifität
    RAG2 Antibody detects endogenous levels of total RAG2.
    Homologie
    Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
    Aufreinigung
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthesized peptide derived from human RAG2, corresponding to a region within N-terminal amino acids.
    Isotyp
    IgG
    Top Product
    Discover our top product RAG2 Primärantikörper
  • Applikationshinweise
    WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at -20 °C. Stable for 12 months from date of receipt.
    Haltbarkeit
    12 months
  • Target
    RAG2 (Recombination Activating Gene 2 (RAG2))
    Andere Bezeichnung
    RAG2 (RAG2 Produkte)
    Synonyme
    RAG-2 antikoerper, Rag-2 antikoerper, rag-2 antikoerper, zgc:136743 antikoerper, recombination activating 2 antikoerper, recombination activating gene 2 antikoerper, recombination activating gene 2 S homeolog antikoerper, RAG2 antikoerper, Rag2 antikoerper, rag2 antikoerper, rag2.S antikoerper
    Hintergrund

    Description: Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3 (By similarity).

    Gene: RAG2

    Molekulargewicht
    59kDa
    Gen-ID
    5897
    UniProt
    P55895
    Pathways
    Chromatin Binding, Production of Molecular Mediator of Immune Response
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