POLD4 Antikörper (Internal Region)

Produktnummer ABIN6264334

Produktdetails anti-POLD4 Antikörper

Target: POLD4 (Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Spezifität: POLD4 Antibody detects endogenous levels of total POLD4.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human POLD4, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu POLD4

Target: POLD4 (Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4))

Andere Bezeichnung: POLD4 (POLD4 Produkte)

Synonyme: 2410012M21Rik antikoerper, AI463381 antikoerper, AW060307 antikoerper, Polds antikoerper, p12 antikoerper, POLDS antikoerper, DNA polymerase delta 4, accessory subunit antikoerper, polymerase (DNA-directed), delta 4 antikoerper, POLD4 antikoerper, Pold4 antikoerper

Hintergrund:

Description: As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity (PubMed:16510448, PubMed:19074196, PubMed:20334433). Pol-delta4 participates in Okazaki fragment processing, through both the short flap pathway, as well as a nick translation system (PubMed:24035200). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb (PubMed:24310611). Involved in Pol-delta4 translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites (PubMed:19074196). Its degradation in response to DNA damage is required for the inhibition of fork progression and cell survival (PubMed:24022480).

Gene: POLD4

Molekulargewicht: 12 kDa

Gen-ID: 57804

UniProt: Q9HCU8

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA