ERN1 Antikörper (Internal Region)

Produktnummer ABIN6262648

Produktdetails anti-ERN1 Antikörper

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Spezifität: IRE1 Antibody detects endogenous levels of total IRE1.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human IRE1, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:1000-3000, IF/ICC 1:200-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Referenzen für anti-ERN1 Antikörper (ABIN6262648)

Lu, Chen, Tang, Wang, Zhang: "GRP78 silencing enhances hyperoxia-induced alveolar epithelial cell apoptosis via CHOP pathway." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 1493-1501, (2018) (PubMed).

Yang, He, Li, Zhang, He, Duan, Huang, Xiang: "SWNHs (Single-Wall Carbon Nanohorns) Supervises Endoplasmic Reticulum (ER) Stress in Hepatocellular Carcinoma." in: Journal of nanoscience and nanotechnology, Vol. 18, Issue 10, pp. 6740-6745, (2018) (PubMed).

Details zu ERN1

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Andere Bezeichnung: ERN1 (ERN1 Produkte)

Synonyme: IRE1 antikoerper, IRE1P antikoerper, IRE1a antikoerper, hIRE1p antikoerper, 9030414B18Rik antikoerper, AI225830 antikoerper, C85377 antikoerper, Ire1a antikoerper, Ire1alpha antikoerper, Ire1p antikoerper, RGD1559716 antikoerper, Ern1 antikoerper, Ire1 antikoerper, Ire1b antikoerper, sb:eu1005 antikoerper, zgc:109932 antikoerper, endoplasmic reticulum to nucleus signaling 1 antikoerper, endoplasmic reticulum to nucleus signaling 2 antikoerper, endoplasmic reticulum (ER) to nucleus signalling 1 antikoerper, serine/threonine-protein kinase/endoribonuclease IRE1 antikoerper, endoplasmic reticulum (ER) to nucleus signalling 2 antikoerper, ERN1 antikoerper, ERN2 antikoerper, Ern1 antikoerper, LOC524719 antikoerper, Ern2 antikoerper, ern1 antikoerper

Hintergrund:

Description: Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11779464, PubMed:11175748, PubMed:12637535, PubMed:9637683, PubMed:21317875). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed:21317875). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11779464, PubMed:24508390, PubMed:21317875). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11779464, PubMed:24508390, PubMed:21317875).

Gene: ERN1

Molekulargewicht: 110 kDa

Gen-ID: 2081

UniProt: O75460

Pathways: ER-Nucleus Signaling, Unfolded Protein Response