THO Complex 4 Antikörper (Internal Region)

Produktnummer ABIN6257532

Produktdetails anti-THO Complex 4 Antikörper

Target: THO Complex 4 (THOC4)

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser THO Complex 4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: THOC4 Antibody detects endogenous levels of total THOC4.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Dog,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human THOC4, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu THO Complex 4

Target: THO Complex 4 (THOC4)

Andere Bezeichnung: ALYREF (THOC4 Produkte)

Synonyme: THOC4 antikoerper, Aly antikoerper, ALY antikoerper, ALY/REF antikoerper, BEF antikoerper, REF antikoerper, tho4 antikoerper, thoc4 antikoerper, zgc:171753 antikoerper, Tho4-A antikoerper, alyref-a antikoerper, thoc4-a antikoerper, REF1 antikoerper, Refbp1 antikoerper, Thoc4 antikoerper, Tho4 antikoerper, Aly/REF export factor antikoerper, THO complex 4 antikoerper, Aly/REF export factor L homeolog antikoerper, ALYREF antikoerper, Thoc4 antikoerper, alyref antikoerper, alyref.L antikoerper, Alyref antikoerper

Hintergrund:

Description: Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production, ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA, both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA, proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability.

Gene: ALYREF

Molekulargewicht: 27 kDa

Gen-ID: 10189

UniProt: Q86V81