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TAG-72 Antikörper

TAG-72 Reaktivität: Human, Ratte, Rind (Kuh), Hund FACS, IF, IHC, WB Wirt: Maus Monoclonal CC49 unconjugated
Produktnummer ABIN6252881
  • Target Alle TAG-72 Antikörper anzeigen
    TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))
    Reaktivität
    • 12
    • 5
    • 4
    • 4
    • 3
    Human, Ratte, Rind (Kuh), Hund
    Wirt
    • 8
    • 4
    Maus
    Klonalität
    • 9
    • 3
    Monoklonal
    Konjugat
    • 9
    • 1
    • 1
    • 1
    Dieser TAG-72 Antikörper ist unkonjugiert
    Applikation
    • 8
    • 7
    • 7
    • 6
    • 3
    • 3
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
    Aufreinigung
    Protein G affinity chromatography
    Immunogen
    Purified TAG-72 protein was used as the immunogen for this TAG-72 antibody.
    Klon
    CC49
    Isotyp
    IgG1
    Top Product
    Discover our top product TAG-72 Primärantikörper
  • Applikationshinweise
    Western blot: 1-2 μg/mL

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 1-2 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

    Prediluted format: incubate for 30 min at RT (2)

    The optimal dilution of the TAG-72 antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.2 mg/mL
    Buffer
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Target
    TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))
    Andere Bezeichnung
    TAG-72 (TAG-72 Produkte)
    Synonyme
    mRNA cap guanine-N7 methyltransferase antikoerper, tag-72 antikoerper
    Hintergrund
    Recognizes an oncofetal antigen of 220  kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This mAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
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