Nuclear Antigen Antikörper (CF®488A)

Produktnummer ABIN6190812

Produktdetails

Target: Nuclear Antigen

Reaktivität: Human, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: CF®488A

Applikation: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Verwendungszweck: Mouse Monoclonal anti-Human Nuclear Antigen (235-1), CF488A Conjugate

Produktmerkmale: This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Nuclei of human myeloid leukemia biopsy cells

Klon: 235-1

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology Frozen 0.5-1.0 μg/mL

• Immunocytochemistry acetone-fixed cells 0.25-0.5 μg/mL
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunoprecipitation 0.5-1 μg/500 μg protein lysate
• Does not react with mouse, rat or chicken, others not known
• Optimal dilution for a specific application should be determined by user

Kommentare:

All human cells. Tonsil

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Antigendetails

Target: Nuclear Antigen

Hintergrund: HNA, HuNu

Molekulargewicht: 70 kDa & 80 kDa