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Maus anti-Human kappa Light Chain Antikörper

FACS, WB, IHC (f), IF Monoclonal L1C1 IgG1 kappa unconjugated
Produktnummer ABIN6174183
  • Target Alle kappa Light Chain Produkte
    kappa Light Chain
    Reaktivität
    • 34
    • 13
    • 2
    • 2
    • 1
    • 1
    Human
    Wirt
    • 20
    • 11
    • 9
    • 4
    • 3
    • 3
    Maus
    Klonalität
    • 26
    • 24
    Monoklonal
    Applikation
    • 33
    • 26
    • 18
    • 18
    • 15
    • 10
    • 9
    • 9
    • 8
    • 6
    • 5
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF)
    Verwendungszweck
    Mouse Monoclonal anti-Human Kappa Light Chain (L1C1), Purified, BSA-free
    Produktmerkmale
    This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Aufreinigung
    Purified
    Immunogen
    Human B-Lymphoma Cells
    Klon
    L1C1
    Isotyp
    IgG1 kappa
  • Applikationshinweise
    Does not react with rat, others not known
    • Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Western blotting 0.5-1 μg/mL
    • Flow Cytometry 0.5-2.0 μg/million cells in 0.1 mL
    • Optimal dilution for a specific application should be determined by user
    Kommentare

    293T, Raji or hPBL cells. Tonsil or Spleen

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS (no BSA, no azide)
    Konservierungsmittel
    Azide free
  • Target
    kappa Light Chain
    Abstract
    kappa Light Chain Produkte
    Hintergrund
    HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM
    Molekulargewicht
    ~22.5 kDa
    Gen-ID
    3514, 449609
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