RFWD2 Antikörper (AA 582-731)

Produktnummer ABIN6146897

Produktdetails anti-RFWD2 Antikörper

Target: RFWD2 (Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2))

Bindungsspezifität: AA 582-731

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFWD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Sequenz: YYDLRNTKQP IMVFKGHRKA VSYAKFVSGE EIVSASTDSQ LKLWNVGKPY CLRSFKGHIN EKNFVGLASN GDYIACGSEN NSLYLYYKGL SKTLLTFKFD TVKSVLDKDR KEDDTNEFVS AVCWRALPDG ESNVLIAANS QGTIKVLELV

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 582-731 of human RFWD2 (NP_071902.2).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB,1:500 - 1:2000,IHC,1:100 - 1:200

Kommentare:

HIGH QUALITY

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Details zu RFWD2

Target: RFWD2 (Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2))

Andere Bezeichnung: RFWD2 (RFWD2 Produkte)

Hintergrund: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53 ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable.,RFWD2,COP1,RNF200,Epigenetics & Nuclear Signaling,Cell Biology & Developmental Biology,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,RFWD2

Molekulargewicht: 14 kDa/57 kDa/77 kDa/78 kDa/80 kDa

Gen-ID: 64326

UniProt: Q8NHY2

Pathways: Photoperiodism