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NF-kB p65 Antikörper

NFkBP65 Reaktivität: Human WB, IHC, IF, IP Wirt: Maus Monoclonal unconjugated
Produktnummer ABIN6135887
  • Target Alle NF-kB p65 (NFkBP65) Antikörper anzeigen
    NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
    Reaktivität
    • 540
    • 374
    • 302
    • 41
    • 38
    • 29
    • 24
    • 22
    • 18
    • 17
    • 14
    • 11
    • 5
    • 2
    • 2
    • 1
    • 1
    Human
    Wirt
    • 492
    • 52
    • 4
    • 2
    • 1
    Maus
    Klonalität
    • 458
    • 92
    Monoklonal
    Konjugat
    • 295
    • 30
    • 23
    • 19
    • 18
    • 17
    • 17
    • 17
    • 17
    • 17
    • 16
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 2
    • 2
    • 1
    Dieser NF-kB p65 Antikörper ist unkonjugiert
    Applikation
    • 427
    • 203
    • 134
    • 118
    • 112
    • 104
    • 92
    • 77
    • 75
    • 29
    • 25
    • 9
    • 7
    • 7
    • 7
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
    Kreuzreaktivität
    Human, Maus, Ratte
    Produktmerkmale
    Monoclonal Antibodies
    Immunogen
    Recombinant protein of human NF-kB p65
    Isotyp
    IgG
    Top Product
    Discover our top product NFkBP65 Primärantikörper
  • Applikationshinweise
    WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:100,IP,1:20 - 1:50
    Kommentare

    HIGH QUALITY

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #104075 (Immunofluorescence)
    'Independent Validation' Siegel
    by
    Klinik für Anästhesiologie, Intensivmedizin und Schmerztherapie des Universitätsklinikums Knappschaftskrankenhaus Bochum
    No.
    #104075
    Datum
    21.11.2019
    Antigen
    NfkBP65
    Chargennummer
    9410609001
    Validierte Anwendung
    Immunofluorescence
    Positivkontrolle

    U-937 human monocytic cell line

    Negativkontrolle

    No primary antibody control

    Bewertung

    Passed. ABIN6135887 specifically stains RelA/p65 in U-937 human monocytic cells with antigen retrieval using 1% SDS.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN6135887
    Sekundärantikörper
    AF488-conjugated AffiniPure alpaca anti-mouse IgG antibody (Jackson Immuno Research, 615-545-214)
    Full Protocol
    • Grow U-937 cells in RPMI 1640 Medium (Gibco, 21875034) supplemented with 10 % FCS (Gibco, A3840001) and Pen/Strep (Gibco, 15140122), at 37°C and 5% CO2 in 300µL in 24-well plates (Sarstedt AG&Co. KG).
    • Use cytospin to spin cells onto slide.
    • Fix cells on coverslide in 4% paraformaldehyde (Carl Roth GmbH, 0335.1) for 30min at RT.
    • Wash cells 3x for 5min with PBS (Sigma-Aldrich, 8537).
    • Permeabilize cells in 100µL PBS containing 0.1 % Triton X-100 (Sigma-Aldrich, T8787) for 5min at RT.
    • Wash cells 3x for 5min with PBS.
    • Permeablilize cells in 100 µL PBS containing 1 % SDS (0183, Carl Roth GmbH) for 5 min at RT.
    • Wash cells 3x for 5 min with PBS.
    • Block non-specific binding with 1x Duolink Blocking solution (Sigma-Aldrich, DUO82007) for 30min at RT.
    • Incubate cells with primary mouse anti-human RelA/p65 antibody (antibodies-online, ABIN6135887, lot 9410609001) diluted 1:100 in 1x Duolink Antibody diluent (Sigma-Aldrich, DUO82008) ON at 4°C.
    • Wash cells 3x for 5min with PBS.
    • Incubate cells with secondary AF488-conjugated AffiniPure alpaca anti-mouse IgG antibody (Jackson Immuno Research, 615-545-214) diluted 1:400 in 1x Duolink Antibody diluent for 1h at RT in the dark.
    • Wash cells 2x for 5min with PBS.
    • Mount coverslips on glass slides in 50µL ProLong Gold antifade mountant with DAPI (Invitrogen, P36931).
    • Image acquisition with an Olympus widefield microscope.
    Anmerkungen

    Strong green staining dependent on the presence of primary antibody indicates that the antibody works. Negative control lacking the primary antibody shows only background fluorescence. Staining was obtained using antigen retrieval (1% SDS). Without this the antibody did not yield detectable signal.

  • Format
    Liquid
    Buffer
    PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at -20°C. Avoid freeze / thaw cycles.
  • Fang, Fang, Ren, Hong, Liu, Zhao, Wang, Peng, Xiao: "Porcine Deltacoronavirus Accessory Protein NS6 Antagonizes Interferon Beta Production by Interfering with the Binding of RIG-I/MDA5 to Double-Stranded RNA." in: Journal of virology, Vol. 92, Issue 15, (2018) (PubMed).

  • Target
    NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
    Andere Bezeichnung
    RELA (NFkBP65 Produkte)
    Synonyme
    C-Rel antikoerper, NFKB3 antikoerper, p65 antikoerper, NFkB antikoerper, c-rel antikoerper, zgc:100833 antikoerper, Xrel2 antikoerper, Xrel3 antikoerper, rel antikoerper, rel-A antikoerper, rel2 antikoerper, rel3 antikoerper, v-rel antikoerper, xrel antikoerper, REL proto-oncogene, NF-kB subunit antikoerper, RELA proto-oncogene, NF-kB subunit antikoerper, v-rel reticuloendotheliosis viral oncogene homolog A (avian) antikoerper, v-rel avian reticuloendotheliosis viral oncogene homolog antikoerper, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog antikoerper, REL antikoerper, RELA antikoerper, Rela antikoerper, Rel antikoerper, rel antikoerper, rel.L antikoerper
    Hintergrund
    NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.,NFKB3,p65,NF-kB p65,RELA,CMCU,Epigenetics & Nuclear Signaling,Transcription Factors,Cancer,Signal Transduction,MAPK-P38 Signaling Pathway,Cell Biology & Developmental Biology,Apoptosis,Inhibition of Apoptosis,Death Receptor Signaling Pathway,Endocrine & Metabolism,Endocrine and metabolic diseases,Obesity,Immunology & Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,IL-6 Receptor Signaling Pathway,NF-kB Signaling Pathway,Toll-like Receptor Signaling Pathway,Neuroscience,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease,Cardiovascular,RELA
    Molekulargewicht
    58 kDa/59 kDa/60 kDa
    Gen-ID
    5970
    UniProt
    Q04206
    Pathways
    NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteine
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