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HIST1H4A Antikörper (acLys5, acLys8, acLys12)

Name: HIST1H4A Antikörper (acLys5, acLys8, acLys12)
SKU: ABIN5773871
Price: 912.45 EUR
Availability: InStock

HIST1H4A Reaktivität: Human, Maus ELISA, WB, IF, ChIP, DB, ChIP-seq Wirt: Kaninchen Polyclonal unconjugated

Western Blot analysis of (1) 25 ug whole cell extracts of Hela cells, (2) 15 ug histone extracts of Hela cells, (3) 1 ug of recombinant histone H2A, (4) 1 ug of recombinant histone H2B, (5) 1 ug of recombinant histone H3, (6) 1 ug of recombinant histone H4.

ChIP was performed on sheared chromatin from 100,000 K562 cells.

ELISA

ChIP assays were performed using human K562 cells.

Immunofluorescent staining of Hela cell line with antibody followed by (A) an anti-rabbit antibody conjugated to Alexa488, (B) antibody after incubation of the antibody following blocking peptides: H4K5,8,12 unmodified, (C) H4K5,8,12ac, (D) H2A.

Cross reactivity test using the Histone H4 (K5,8,12ac) antibody.

6 Abbildungen

Produktnummer ABIN5773871

Datenblatt (PDF)

Target Alle HIST1H4A Antikörper anzeigen

HIST1H4A (Histone Cluster 1, H4a (HIST1H4A))
Bindungsspezifität
10

8

7

6

6

6

6

5

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
acLys5, acLys8, acLys12
Reaktivität
102

17

8

2

2

1

1

1
Human, Maus
Wirt
98

5
Kaninchen
Passende Sekundärantikörper
Klonalität
87

16
Polyklonal
Konjugat
94

3

2

2

1

1
Dieser HIST1H4A Antikörper ist unkonjugiert
Applikation
80

60

34

32

29

23

18

15

11

10

4

3

1

1

1
ELISA, Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Verwendungszweck

Rabbit polyclonal antibody raised against synthetic peptide of Histone H4 (K5,8,12ac).

Kreuzreaktivität

Human, Maus

Immunogen

A synthetic peptide (conjugated with KLH) corresponding to Histone H4, acetylated at lysines 5, 8, and 12.
anti-Histone Cluster 1, H4a (HIST1H4A) (3meLys20) antibody
HIST1H4A Reaktivität: Human, Maus ELISA, WB, IF, ChIP, DB, ChIP-seq Wirt: Kaninchen Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (acLys5) antibody
HIST1H4A Reaktivität: Human, Maus ELISA, WB, IF, ChIP, DB, ChIP-seq Wirt: Kaninchen Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (acLys12), (acLys16), (acLys5), (acLys8) antibody
HIST1H4A Reaktivität: Human, Maus ELISA, IF, ChIP, DB, ChIP-seq Wirt: Kaninchen Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (acLys8) antibody
HIST1H4A Reaktivität: Human, Maus ELISA, WB, IF, ChIP, DB Wirt: Kaninchen Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (meLys20) antibody
HIST1H4A Reaktivität: Human ELISA, WB, IHC, DB, FACS Wirt: Kaninchen Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (acLys31) antibody
HIST1H4A Reaktivität: Human ELISA, WB, IF, ChIP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (acLys16) antibody
HIST1H4A Reaktivität: Human ELISA, WB, IF, ChIP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (acLys16) antibody
HIST1H4A Reaktivität: Human ELISA, WB, IF, ICC, FACS Wirt: Kaninchen Monoclonal 2B8 unconjugated Recombinant Antibody

anti-Histone Cluster 1, H4a (HIST1H4A) (2meLys20), (AA 1-30) antibody
HIST1H4A Reaktivität: Human WB, DB, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB17115 unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (acLys12) antibody
HIST1H4A Reaktivität: Human ELISA, IF, ChIP, ICC Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

ELISA (1:1000)
Western Blot (1:1000)
ChIP (0.5-1 μg/IP)
Dot Blot (1:20000)
Immunofluorescence (1:500)
The optimal working dilution should be determined by the end user.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

In PBS (0.05 % sodium azide, 0.05 % proclin 300).

Konservierungsmittel

ProClin, Sodium azide

Vorsichtsmaßnahmen

This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung

-20 °C,-80 °C

Informationen zur Lagerung

Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
Target

HIST1H4A (Histone Cluster 1, H4a (HIST1H4A))

Andere Bezeichnung

HIST1H4A (HIST1H4A Produkte)

Synonyme

Hist1h4a antikoerper, H4FA antikoerper, histone cluster 1, H3f antikoerper, histone cluster 1 H4 family member a antikoerper, histone cluster 1, H4a antikoerper, Hist1h3f antikoerper, HIST1H4A antikoerper, Hist1h4a antikoerper

Hintergrund

Full Gene Name: histone cluster 1, H4a
Synonyms: H4/a,H4FA

Gen-ID

8359