CaMKII alpha/beta (Internal Region) Antikörper
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- Target
- CaMKII alpha/beta
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Bindungsspezifität
- Internal Region
- Reaktivität
- Ratte, Human, Maus
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Unkonjugiert
- Applikation
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
- Verwendungszweck
- CAMK2
- Sequenz
- PRTAQSEETR VWHR
- Spezifität
- This antibody is expected to recognize both reported isoforms (NP_057065.2 (CAMK2A) and NP_001280099.1 (CAMK2B).
- Kreuzreaktivität
- Human, Maus, Ratte
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- Peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2, NP_741960.1.
- Isotyp
- IgG
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- Applikationshinweise
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Western Blot: Approx 48-50+60 kDa bands observed in Human Cerebral Cortex and Mouse and Rat Brain lysates, corresponding to CAMK2alpha and CAMK2beta respectively and approx. 60 kDa in Human Cerebellum lysates (calculated MW of 55.3 kDa according to Human NP
Peptide ELISA: antibody detection limit dilution 1:128000.
- Kommentare
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of Neuro2a cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Neuro2a cells. Recommended concentration: 10u
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- CaMKII alpha/beta
- Andere Bezeichnung
- CAMK2A/B
- Hintergrund
- CAMK2A, calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, CAMKA, KIAA0968, CaM kinase II alpha subunit, CaM-kinase II alpha chain, CaMK-II alpha subunit, OTTHUMP00000165787, OTTHUMP00000165788, calcium/calmodulin-dependent protein kinase
- Gen-ID
- 815, 816, 12322
- NCBI Accession
- NP_057065, NP_741960
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