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Histone H4 Antikörper (2meLys20)

Reaktivität: Human, Maus, Ratte, Huhn, Xenopus laevis, Drosophila melanogaster, C. elegans, Pflanzen WB, IHC, ChIP, FM Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN5706798
  • Target Alle Histone H4 Antikörper anzeigen
    Histone H4
    Bindungsspezifität
    • 36
    • 33
    • 31
    • 27
    • 19
    • 19
    • 17
    • 15
    • 14
    • 14
    • 12
    • 12
    • 12
    • 10
    • 9
    • 9
    • 9
    • 9
    • 9
    • 7
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    2meLys20
    Reaktivität
    • 322
    • 258
    • 253
    • 24
    • 14
    • 13
    • 12
    • 11
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    Human, Maus, Ratte, Huhn, Xenopus laevis, Drosophila melanogaster, C. elegans, Pflanzen
    Wirt
    • 359
    • 23
    • 2
    Kaninchen
    Klonalität
    • 331
    • 52
    • 1
    Polyklonal
    Konjugat
    • 213
    • 21
    • 19
    • 19
    • 19
    • 19
    • 19
    • 19
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    Dieser Histone H4 Antikörper ist unkonjugiert
    Applikation
    • 211
    • 102
    • 95
    • 94
    • 55
    • 52
    • 48
    • 41
    • 40
    • 39
    • 34
    • 22
    • 13
    • 9
    • 9
    • 6
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)
    Produktmerkmale

    Synonyms: rabbit anti-Histone H4 dimethyl Lys20 antibody, H4K20me2, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4

    Background: When K20 is di-methylated on H4, the downstream effect is silencing of genes. This modification is also necessary and sufficient for 53BP1 binding, which is a prerequisite for DNA repair, a highly conserved mechanism. H4K20me2 is associated with hypoacetylation, and inactivation of certain genes. NSD1 and Suv420h1/2 trigger the dimethylation of K20, but only after SET8 catalyzes the mono-methylation of the same lysine, indicating a complex regulation of this modification that can so seriously affect replication and stability of genomic information. Anti-Histone H4 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, DNA Repair, DNA replication Transcription Translation and Splicing, Histones and Modified Histones, and Epigenetics research.

    Gene Name: HIST4H4

    Aufreinigung
    Anti-Histone H4 [Dimethyl Lys20] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with most mammal species. Cross-reactivity with Histone H4 from other sources has not been determined.
    Immunogen

    Immunogen: Histone H4 [Dimethyl Lys20] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 20 of human Histone H4.

    Immunogen Type: Peptide

    Isotyp
    IgG
    Top Product
    Discover our top product Histone H4 Primärantikörper
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:50

    Application Note: Anti-Histone H4 [Dimethyl Lys20] antibody is useful for Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunofluorescence, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

    ChIP Dilution: 2-5 μg/million cells

    Western Blot Dilution: 1:2000

    IF Microscopy Dilution: 1:50

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.53 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

    Stabilizer: 30 % Glycerol

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Haltbarkeit
    12 months
  • Target
    Histone H4
    Abstract
    Histone H4 Produkte
    Synonyme
    hist1h4l antikoerper, H1ft antikoerper, Hist4 antikoerper, Hist4h4 antikoerper, H4 antikoerper, X04652 antikoerper, FO108 antikoerper, H4/n antikoerper, H4F2 antikoerper, H4FN antikoerper, HIST2H4 antikoerper, H4C13 antikoerper, H4C7 antikoerper, F8N16.2 antikoerper, F8N16_2 antikoerper, histone H4 antikoerper, BcDNA:RH52884 antikoerper, CG3379 antikoerper, Dmel\CG3379 antikoerper, H4r antikoerper, His4-88CD antikoerper, His4R antikoerper, histone cluster 1, H4a L homeolog antikoerper, histone cluster 1 H4 family member B antikoerper, histone cluster 2, H4 antikoerper, histone cluster 2 H4 family member a antikoerper, histone H4 antikoerper, Histone H4 replacement antikoerper, Histone H4 antikoerper, hist1h4a.L antikoerper, Hist1h4b antikoerper, Hist2h4 antikoerper, HIST2H4A antikoerper, H4 antikoerper, H4C14 antikoerper, HIS4 antikoerper, HIST2H4 antikoerper, His4r antikoerper, his-38 antikoerper
    Gen-ID
    121504
    NCBI Accession
    NP_001029249
    UniProt
    P62805
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