USP28 Antikörper

Produktnummer ABIN5701387

Produktdetails anti-USP28 Antikörper

Target: USP28 (Ubiquitin Specific Peptidase 28 (USP28))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser USP28 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Immunogen: ubiquitin specific peptidase 28

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB : 1:500-1:5000,IP:1:200-1:2000,IHC:1:20-1:200

Kommentare:

HeLa cells were subjected to SDS PAGE followed by western blot with FNab09322(USP28 antibody) at dilution of 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Haltbarkeit: 12 months

Details zu USP28

Target: USP28 (Ubiquitin Specific Peptidase 28 (USP28))

Andere Bezeichnung: USP28 (USP28 Produkte)

Synonyme: im:7138195 antikoerper, USP28 antikoerper, 9830148O20Rik antikoerper, AU022237 antikoerper, mKIAA1515 antikoerper, ubiquitin specific peptidase 28 antikoerper, USP28 antikoerper, usp28 antikoerper, Usp28 antikoerper

Hintergrund: Synonyms:Deubiquitinating enzyme 28, KIAA1515, Ubiquitin thiolesterase 28, USP28 Background:Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome(APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7(FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7(FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes(TSGs) in a subset of colorectal cancers(CRC) cells(PubMed:24623306).

Molekulargewicht: 125kDa

Gen-ID: 57646

UniProt: Q96RU2