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Kaninchen anti-Meerschweinchen IgG (Heavy & Light Chain) Antikörper

IP Polyclonal unconjugated
Produktnummer ABIN457767
  • Target Alle IgG Produkte
    IgG
    Bindungsspezifität
    • 2333
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reaktivität
    • 695
    • 672
    • 561
    • 458
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
    • 97
    • 95
    • 92
    • 62
    • 47
    • 38
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Meerschweinchen
    Wirt
    • 1730
    • 1474
    • 539
    • 206
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Kaninchen
    Klonalität
    • 3361
    • 167
    • 1
    Polyklonal
    Applikation
    • 1840
    • 1800
    • 1792
    • 1501
    • 962
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 52
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunoprecipitation (IP)
    Spezifität
    Precipitating polyclonal Rabbit antiSerum to Guinea Pig IgG, heavy and light chains.
    Kreuzreaktivität (Details)
    Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion with the following results: Bovine ± Cat ± Chicken - Dog ± Goat ± Hamster + Horse ± Human ± Mouse ± Monkey ± pigeon ± Rat + Sheep - Swine - Turkey -
    Produktmerkmale
    The reactivity of the antiserum is directed to the Fc and Fab subunits of both subclasses, IgG1 and IgG2. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against guinea pig serum a single characteristic precipitin line with a spur is usually obtained, representing primarily IgG2. If the IgG1 level in the sample approaches the concentration of IgG2, it may become visible as a second precipitin line. It does not react with any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal guinea pig serum, to prepare an immunoadsorbent for the production of Ig-free guinea pig serum, plasma or non-Ig preparations. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay reagents meeting the necessary additional specificity and performance requirements are available.
    Aufreinigung
    Delipidated, heat inactivated, whole serum
    Sterilität
    Heat-inactivated
    Immunogen
    Purified normal IgG isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
  • Applikationshinweise
    In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    It is reconstituted by adding 1 mL sterile distilled water.Delipidated, heat inactivated, lyophilized whole antiserum. No preservative added, as it may interfere with the antibody activity.No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. No foreign proteins added. It is reconstituted by adding 1 mL sterile distilled water.
    Buffer
    Delipidated, heat inactivated, lyophilized whole antiserum. No preservative added, as it may interfere with the antibody activity.No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. No foreign proteins added.
    Konservierungsmittel
    Without preservative
    Lagerung
    RT,4 °C,-20 °C
    Informationen zur Lagerung
    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
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