Defensin beta 3 Antikörper (Biotin)

Produktnummer ABIN5690603

Produktdetails anti-Defensin beta 3 Antikörper (Biotin)

Target: Defensin beta 3 (DEFB3)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Defensin beta 3 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-3. Human BD-3 specific antibody was purified by affinity chromatography and then biotinylated.

Anwendungsinformationen

Applikationshinweise: ELISA:
Direct:
To detect hBD-3 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-3.

Sandwich
To detect hBD-3 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human BD-3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-3.

Western Blot:
To detect hBD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: BD-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu Defensin beta 3

Target: Defensin beta 3 (DEFB3)

Andere Bezeichnung: BD-3 (DEFB3 Produkte)

Gen-ID: 55894

UniProt: P81534

Pathways: Production of Molecular Mediator of Immune Response