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Estrogen Receptor alpha Antikörper (AA 2-185)

Name: Estrogen Receptor alpha Antikörper (AA 2-185)
Brand: antibodies-online
SKU: ABIN5542594
Price: 614.71 EUR
Availability: InStock

ESR1 Reaktivität: Human WB, IHC, ELISA, FACS, ICC Wirt: Maus Monoclonal 6A6F3 unconjugated

Western blot analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using ESR1 mouse mAb against MOLT4 (1), Raji (2), MCF-7 (3), T47D (4), SK-Br-3 (5), and Hela (6) cell lysate.

Flow cytometric analysis of Hela cells using ESR1 mouse mAb (green) and negative control (red).

FACS

Immunofluorescence analysis of Hela cells using ESR1 mouse mAb.

Immunofluorescence analysis of Hela cells using ESR1 mouse mAb (green).

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using ESR1 mouse mAb with DAB staining.

IHC

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng)

ELISA

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ESR1 mouse mAb with DAB staining.

IHC

Western blot analysis using ESR1 mAb against human ESR1 (AA: 2-185) recombinant protein.

9 Abbildungen

Produktnummer ABIN5542594

Datenblatt (PDF)

Target Alle Estrogen Receptor alpha (ESR1) Antikörper anzeigen

Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))
Bindungsspezifität
32

30

25

23

21

21

21

16

16

16

16

16

15

15

13

12

11

10

9

9

8

7

7

7

6

6

6

4

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2
AA 2-185
Reaktivität
481

173

167

19

18

10

7

6

5

4

3

3

3

2

1

1
Human
Wirt
392

97

2

1
Maus
Passende Sekundärantikörper
Klonalität
372

122
Monoklonal
Konjugat
223

35

24

22

19

15

15

15

15

15

15

11

8

8

8

8

8

4

4

4

4

4

4

1

1

1

1

1

1
Dieser Estrogen Receptor alpha Antikörper ist unkonjugiert
Applikation
377

172

142

120

97

94

80

67

40

38

24

15

10

7

7

6

5

2

2

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Immunogen

Purified recombinant fragment of human ESR1 (AA 2-185) expressed in E. Coli.

Klon

6A6F3

Isotyp

IgG1

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Applikationshinweise

ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

PBS with 0.05 % sodium azide

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

4°C, -20°C for long term storage
Target

Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Andere Bezeichnung

ESR1 (ESR1 Produkte)

Hintergrund

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined.,

Molekulargewicht

58.8kDa

HGNC

4286

Pathways

Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly