ATP1B1 Antikörper (ATPase, Na+/K+ Transporting, beta 1 Polypeptide) (Subunit beta)

Details for Product anti-ATP1B1 Antibody No. ABIN5684017, Anbieter: Anmelden zum Anzeigen
Antigen
  • ATP1B
  • ATPBS
  • Atp4b
  • Atpb
  • Atpb-1
  • NKbeta1
  • ATPase beta chain
  • ATP synthase CF1 beta subunit
  • ATPase Na+/K+ transporting subunit beta 1
  • ATPase, Na+/K+ transporting, beta 1 polypeptide
  • atpB
  • ATP1B1
  • Atp1b1
Epitop
Subunit beta
19
10
8
7
6
4
4
3
2
2
1
1
1
1
1
1
1
1
Reaktivität
Arabidopsis thaliana, Bacillus cereus, Barley (Hordeum vulgare), Huhn, Chlamydomonas reinhardtii (C. reinhardtii), Nicotiana benthamiana, Schwein, Ratte (Rattus), Reis (Oryza sativa), Lachs (Salmonidae), Sojabohne (Glycine max), Spinat (Spinacia oleracea), Tabak (Nicotiana tabacum), Zea mays subsp. parviglumis (Balsas teosinte)
79
43
38
11
9
7
6
5
4
4
4
2
2
2
1
1
1
1
1
Wirt
Kaninchen
71
18
7
2
Klonalität
Polyklonal
Konjugat
Dieser ATP1B1 Antikörper ist unkonjugiert
6
6
5
3
3
3
Applikation
Blue-native PAGE (BN PAGE), Immunofluorescence (IF), Western Blotting (WB)
91
53
51
16
10
7
7
6
3
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3
Spezifität The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 ATP synthase and most known bacterial F-type ATP synthases. Peptide used for antibody production is located ina beta sheet, which is partly exposed near the surface of the AtpB protein.
Kreuzreaktivität (Details) No cross-reactivity with: archeal V-type ATP synthase
Produktmerkmale Expected / apparent Molecular Weight of the Antigene: 53.9kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)
Reinigung serum
Plasmids, Primers & others Plasmide, Primers & weitere ATP1B1 products on genomics-online (e.g. as negative or positive controls)
Andere Bezeichnung AtpB (ATP1B1 Antibody Abstract)
Hintergrund AGI Code: At5g08670, ATCG00480
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
Molekulargewicht 53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)
UniProt P83483, A8IQU3, P19366, P06541
Pathways Thyroid Hormone Synthesis, Ribonucleoside Biosynthetic Process
Applikations-hinweise Recommended Dilution: 1 : 2000 - 1 : 5 000 with standard ECL (WB), 1 : 5000 (BN-PAGE).
Kommentare

Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution For reconstitution add 10 µL of sterile water
Handhabung Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Lagerung -20 °C
Bilder des Herstellers
Western Blotting (WB) image for anti-ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1) (Subunit beta) antibody (ABIN5684017) 2 µg of total protein extracted with PEB from leaf tissue of (1) Arabidopsis thalian...
 image for anti-ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1) (Subunit beta) antibody (ABIN5684017) 2 µg of total protein from (1) cow, (2) chicken, (3) pig, (4) rat, (5) salmon, (6) s...
Produkt verwendet in: Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, 2015 (PubMed).

Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." in: Journal of plant physiology, 2011 (PubMed).

Berto, DAdamo, Bergantino, Vallese, Giacometti, Costantini: "The cyanobacterium Synechocystis sp. PCC 6803 is able to express an active [FeFe]-hydrogenase without additional maturation proteins." in: Biochemical and biophysical research communications, 2011 (PubMed).

Ahsan, Nakamura, Komatsu: "Differential responses of microsomal proteins and metabolites in two contrasting cadmium (Cd)-accumulating soybean cultivars under Cd stress." in: Amino acids, 2010 (PubMed).

Allgemeine Veröffentlichungen Lintala, Schuck, Thormählen, Jungfer, Weber, Weber, Geigenberger, Soll, Bölter, Mulo: "Arabidopsis tic62 trol mutant lacking thylakoid-bound ferredoxin-NADP+ oxidoreductase shows distinct metabolic phenotype." in: Molecular plant, Vol. 7, Issue 1, pp. 45-57, 2014 (PubMed).

Eom, Lee, Choi: "Bacillus subtilis HJ18-4 from traditional fermented soybean food inhibits Bacillus cereus growth and toxin-related genes." in: Journal of food science, Vol. 79, Issue 11, pp. M2279-87, 2014 (PubMed).

Rasala, Barrera, Ng, Plucinak, Rosenberg, Weeks, Oyler, Peterson, Haerizadeh, Mayfield: "Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii." in: The Plant journal : for cell and molecular biology, Vol. 74, Issue 4, pp. 545-56, 2013 (PubMed).

Heinnickel, Alric, Wittkopp, Yang, Catalanotti, Dent, Niyogi, Wollman, Grossman: "Novel thylakoid membrane GreenCut protein CPLD38 impacts accumulation of the cytochrome b6f complex and associated regulatory processes." in: The Journal of biological chemistry, Vol. 288, Issue 10, pp. 7024-36, 2013 (PubMed).

Teng, Dang, Danne, Waller, Green: "Mitochondrial Genes of Dinoflagellates Are Transcribed by a Nuclear-Encoded Single-Subunit RNA Polymerase." in: PLoS ONE, Vol. 8, Issue 6, pp. e65387, 2013 (PubMed).

Brown, Campbell, Lawrence: "Resource dynamics during infection of Micromonas pusilla by virus MpV-Sp1." in: Environmental microbiology, Vol. 9, Issue 11, pp. 2720-7, 2007 (PubMed).

Haben Sie etwas anderes gesucht?