NFATC1 Antikörper (AA 110-210) (FITC)

Produktnummer ABIN5651056

Produktdetails anti-NFATC1 Antikörper (FITC)

Target: NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))

Bindungsspezifität: AA 110-210

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFATC1 Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Spezifität: Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. Isoforms IA are widely expressed but not detected in liver nor pancreas, neural expression is strongest in corpus callosum. Isoforms IB are expressed mostly in muscle, cerebellum, placenta and thymus, neural expression in fetal and adult brain, strongest in corpus callosum.,Detects 90 kDa, band at ~60 kDa is another isoform.

Kreuzreaktivität: Human, Ratte

Aufreinigung: Peptide Affinity Purified

Immunogen: Synthetic peptide of Human NFATC1 (110-210 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:50)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN5651056 was sufficient for detection of NFATC1 in 15 μg of rat brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu NFATC1

Target: NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))

Andere Bezeichnung: NFATC1 (NFATC1 Produkte)

Gen-ID: 4772

NCBI Accession: NP_001265598

UniProt: O95644

Pathways: RTK Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung