POLA1 Antikörper (AA 1-33)

Produktnummer ABIN5646962

Produktdetails anti-POLA1 Antikörper

Target: POLA1 (DNA Polymerase alpha (POLA1))

Bindungsspezifität: AA 1-33

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Kreuzreaktivität (Details): Expected species reactivity: Mouse,Rat

Aufreinigung: Antigen affinity purified

Immunogen: A portion of amino acids 1-33 from human POLA1 was used as the immunogen for the DNA Polymerase alpha antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the DNA Polymerase alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. WB: 1:1000-1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the DNA Polymerase alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu POLA1

Target: POLA1 (DNA Polymerase alpha (POLA1))

Andere Bezeichnung: DNA Polymerase alpha / POLA1 (POLA1 Produkte)

Synonyme: pola antikoerper, AW321876 antikoerper, Pola antikoerper, POLA antikoerper, p180 antikoerper, polymerase (DNA directed), alpha 1, catalytic subunit S homeolog antikoerper, polymerase (DNA directed), alpha 1 antikoerper, DNA polymerase alpha 1, catalytic subunit antikoerper, pola1.S antikoerper, Pola1 antikoerper, POLA1 antikoerper

Hintergrund: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

UniProt: P09884

Pathways: SARS-CoV-2 Protein Interaktom