Ziege anti-Frettchen IgG (Heavy Chain) Antikörper (Biotin) - Preadsorbed

Produktnummer ABIN5608045

Produktdetails

Target: IgG

Bindungsspezifität: Heavy Chain

Reaktivität: Frettchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Produktmerkmale: Anti-FERRET IgG (gamma chain) (GOAT) Antibody generated in goat detects specifically Ferret IgG gamma chain. Ferret antibody is ideal for investigators involved in Serum Protein Component research.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Ferret IgG gamma heavy chain

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-FERRET IgG (gamma chain) (GOAT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Jackson-Thompson, Kim, Jaiswal, Scott, Jones, Morris, Fite, Laurie, Hoy, Dardzinski, Mitre: "Brugia malayi infection in ferrets - A small mammal model of lymphatic filariasis." in: PLoS neglected tropical diseases, Vol. 12, Issue 3, pp. e0006334, (2018) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: goat anti-Ferret IgG Antibody Biotin Conjugation, goat anti-Ferret IgG Biotin Conjugated Antibody

Background: Anti-Ferret IgG Biotin antibody generated in goat detects ferret IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.