Myosin Antikörper (N-Term, pSer19, pSer20)

Produktnummer ABIN5596872

Produktdetails anti-Myosin Antikörper

Target: Myosin

Bindungsspezifität: N-Term, pSer19, pSer20

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Myosin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Kreuzreaktivität (Details): Anti-Myosin pS19/pS20 antibody is directed against the regulatory light chain of smooth and non-muscle myosin. This antiserum is phosphospecific and detects monophosphorylated and diphosphorylated forms of the protein. Reactivity with non-phosphorylated myosin light chain is less than 1% by ELISA. Cross reactivity is expected with myosin light chain from human and mouse. Reactivity with the protein from other species has not been determined. However, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.

Immunogen:

Immunogen: Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Peptide

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 2.5 μg/mL

Application Note: Anti-Myosin pS19/pS20 phospho specific polyclonal antibody was tested by ELISA, immunohistochemistry and immunoblotting. Immunoblotting was used to show reactivity with unstimulated and stimulated cardiac myocytes. The antibody was also reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional by immunoprecipitation.

Western Blot Dilution: 1:1,000 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 70 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Myosin

Target: Myosin

Abstract: Myosin Produkte

Synonyme: CG3201 antikoerper, DmMLC_C antikoerper, Dmel\\CG3201 antikoerper, MyoII antikoerper, mlc-c antikoerper, nmELC antikoerper, MYOSIN antikoerper, myosin 1 antikoerper, DFNA4 antikoerper, DFNA4A antikoerper, MHC16 antikoerper, MYH17 antikoerper, NMHC II-C antikoerper, NMHC-II-C antikoerper, PNMHH antikoerper, myosin antikoerper, MYH1 antikoerper, MYH2 antikoerper, MYH3 antikoerper, MYH4 antikoerper, MYH8 antikoerper, adult antikoerper, embryonic antikoerper, 2400004E04Rik antikoerper, II-C antikoerper, NHMCII antikoerper, DROMHC antikoerper, Dm nmII antikoerper, DmnmII antikoerper, Dronm-MII antikoerper, E(br) antikoerper, MHC antikoerper, Mhc-c antikoerper, Mhc-c[1] antikoerper, Myo antikoerper, Myo II antikoerper, NMM antikoerper, Zip antikoerper, anon-WO0140519.37 antikoerper, l(2)02957 antikoerper, nmMHC antikoerper, nmy-2 antikoerper, zpr antikoerper, DmelCG15792 antikoerper, CG15792 antikoerper, Myosin light chain cytoplasmic antikoerper, hypothetical protein antikoerper, myosin 1 antikoerper, myosin heavy chain 9 antikoerper, myosin heavy chain 14 antikoerper, myosin, heavy chain 1B, skeletal muscle antikoerper, myosin, heavy polypeptide 14 antikoerper, zipper antikoerper, Mlc-c antikoerper, T22C1.6 antikoerper, ATM1 antikoerper, MYH9 antikoerper, MYH14 antikoerper, Myh14 antikoerper, MYH1B antikoerper, zip antikoerper

Hintergrund:

Synonyms: Myosin regulatory light chain 12A, Myosin regulatory light chain MRLC3, Myosin regulatory light chain 2, nonsarcomeric, Myosin RLC, MLC-2B,

Background: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium levels and myosin phosphorylation, resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. This antibody is specific for the phosphorylated form of myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human and mouse have almost identical sequences. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.

Gene Name: MYL12A

Gen-ID: 10627

UniProt: P19105