CDKN1B Antikörper (Internal Region, pSer140)
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- Target Alle CDKN1B Antikörper anzeigen
- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
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Bindungsspezifität
- Internal Region, pSer140
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser CDKN1B Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Kreuzreaktivität (Details)
- This antibody will specifically react with a p27kip1 protein from human tissue. Reactivity against p27 from other species is unknown.
- Aufreinigung
- Anti-p27 kip1 pS140 is directed against the phosphorylated form of human p27 kip1 protein at the S140 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.
- Immunogen
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Immunogen: Anti-p27 kip1 pS140 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to S140 residue of human p27 kip1 protein.
Immunogen Type: Peptide
- Isotyp
- IgG
- Top Product
- Discover our top product CDKN1B Primärantikörper
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- Applikationshinweise
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-p27kip1 pS140 antibody is suitable for use in ELISA, immunoprecipitation, co-immunoprecipitation and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~27 kDa in size corresponding to p27 by western blotting in the appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human p27. Optimal titers for other applications should be determined by the researcher.
Western Blot Dilution: 1:500 - 1:4,000
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:10,000 - 1:40,000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.68 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
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Upregulation of human glycolipid transfer protein (GLTP) induces necroptosis in colon carcinoma cells." in: Biochimica et biophysica acta. Molecular and cell biology of lipids, Vol. 1864, Issue 2, pp. 158-167, (2019) (PubMed).
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Upregulation of human glycolipid transfer protein (GLTP) induces necroptosis in colon carcinoma cells." in: Biochimica et biophysica acta. Molecular and cell biology of lipids, Vol. 1864, Issue 2, pp. 158-167, (2019) (PubMed).
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- Target
- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
- Andere Bezeichnung
- p27 kip1 (CDKN1B Produkte)
- Synonyme
- CDKN4 antikoerper, KIP1 antikoerper, MEN1B antikoerper, MEN4 antikoerper, P27KIP1 antikoerper, cdkn1b antikoerper, sb:cb611 antikoerper, wu:fb63g10 antikoerper, wu:fb64g10 antikoerper, wu:fe18e03 antikoerper, CDKN1B antikoerper, Kip1 antikoerper, p27 antikoerper, FAM14D antikoerper, ISG12 antikoerper, ISG12A antikoerper, P27 antikoerper, Cdki1b antikoerper, AA408329 antikoerper, AI843786 antikoerper, p27Kip1 antikoerper, cdkn1bl antikoerper, cyclin dependent kinase inhibitor 1B antikoerper, cyclin-dependent kinase inhibitor 1Bb antikoerper, interferon alpha inducible protein 27 antikoerper, cyclin-dependent kinase inhibitor 1B antikoerper, cyclin-dependent kinase inhibitor 1Ba antikoerper, CDKN1B antikoerper, cdkn1bb antikoerper, IFI27 antikoerper, Cdkn1b antikoerper, cdkn1ba antikoerper
- Hintergrund
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Synonyms: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1,
Background: p27 kip1 pS140 is a cyclin-dependent kinase inhibitor that shares a limited similarity with CDK inhibitor CDKN1A/p21. p27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Phosphorylation occurs on serine, threonine and tyrosine residues. Anti-p27 kip1 pS140 antibody is ideal for researchers interested in cell cycle and cancer research.
Gene Name: CDKN1B
- Gen-ID
- 1027
- UniProt
- P46527
- Pathways
- Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagie
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