CNST Antikörper
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- Target Alle CNST Antikörper anzeigen
- CNST (Consortin, Connexin Sorting Protein (CNST))
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Reaktivität
- Maus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser CNST Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB)
- Verwendungszweck
- Rabbit polyclonal antibody raised against partial recombinant Cnst.
- Sequenz
- MAPEERRDSE DRVSKETEDY LHSLLERCLK DAEDSLSYED IQDDDSDLLQ DLSPEEASYS LQEDLPPDES TLSLDDLAKK IEIAEAIPAE GLVSILKKRN DTVGSHPAQM QQKPAKRRVR FQEID
- Spezifität
- Specific to recombinant protein GX2268. This antibody detects mC1orf71 protein.
- Kreuzreaktivität
- Maus
- Immunogen
- Recombinant GST fusion protein corresponding to 125 amino acids of mouse Cnst.
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- Applikationshinweise
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Western Blot (1:1000)
The optimal working dilution should be determined by the end user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- by
- Christoffels Lab, Heart Failure Research Center, Academisch Medisch Centrum Amsterdam
- No.
- #102674
- Datum
- 14.05.2018
- Antigen
- CNST
- Chargennummer
- BIAD001
- Validierte Anwendung
- Western Blotting
- Positivkontrolle
- Whole E13.5 wildtype mouse embryo protein lysate
- Negativkontrolle
- Whole E13.5 mouse embryo protein lysates homozygous for a null Cnst allele. This mouse line was generated using CRISPR/Cas9.
- Bewertung
Passed. ABIN5575708 specifically recognizes Cnst in whole embryo lysates.
- Primärantikörper
- ABIN5575708
- Sekundärantikörper
- anti-rabbit HRP-conjugated antibody (GE Healthcare, NA934V, lot 9804671)
- Full Protocol
- Isolate E13.5 mouse embryos in cold PBS and snap freeze with liquid nitrogen.
- Lyse each individual embryo in 300µl of cold RIPA buffer.
- Determine total protein content of the lysates using Pierce™ BCA Protein Assay Kit (Thermo Scientific, 23227, lot NA168207).
- Denature 5µg of total protein for 5min at 95°C in 20µl 2x Laemmli SDS buffer and subsequently separate them on a 10% denaturing acrylamide gel in a BIO-RADMini-PROTEAN 3 chamber for 1h at 180V.
- Transfer proteins onto PVDF membrane (Immobilon-P, Millipore) with a Western blotting system for 45min at 25V.
- Block the membrane with 5% Milk in TBST as blocking buffer for 1-2h at RT.
- Incubation with primary
- rabbit anti-Consortin antibody (antibodies-online, ABIN5575708, lot BIAD001) diluted 1:2000 in blocking buffer 5% milk ON at 4°C.
- mouse anti-GAPDH antibody (Santa Cruz, sc-365062, lot I0916) diluted 1:5000 in blocking buffer ON at 4°C.
- Wash membrane 5x for 15min with TBST.
- Incubation with secondary
- anti-rabbit HRP-conjugated antibody (GE Healthcare, NA934V, lot 9804671) diluted 1:2000 blocking buffer for 1-2h at RT.
- anti-mouse HRP-conjugated antibody (GE Healthcare, NA931V, lot 9800151) diluted 1:2000 in blocking buffer for 1-2h at RT.
- Wash membrane 5x for 15min with TBST.
- Reveal protein bands using ECL detection kit (GE Healthcare) on an ImageQuant LAS 4000 phosphoimaging unit (GE Healthcare).
- Anmerkungen
The Consortin antibody ABIN5575708 reveals a protein of the expected molecular weight of antigen in lysates of whole E13.5 embryos. The protein bands is only visible in the positive but not the negative controls.
Validierung #102674 (Western Blotting)ValidierungsbilderProtokoll -
- Format
- Liquid
- Buffer
- In PBS (50 % glycerol, 0.02 % sodium azide)
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
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Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
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- Target
- CNST (Consortin, Connexin Sorting Protein (CNST))
- Andere Bezeichnung
- 9630058J23Rik (CNST Produkte)
- Synonyme
- C1orf71 antikoerper, PPP1R64 antikoerper, 9630058J23Rik antikoerper, consortin, connexin sorting protein antikoerper, CNST antikoerper, Cnst antikoerper
- Hintergrund
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Full Gene Name: RIKEN cDNA 9630058J23 gene
Synonyms: MGC31547 - Gen-ID
- 226744
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