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CNST Antikörper

CNST Reaktivität: Maus WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN5575708
  • Target Alle CNST Antikörper anzeigen
    CNST (Consortin, Connexin Sorting Protein (CNST))
    Reaktivität
    • 8
    • 1
    Maus
    Wirt
    • 8
    Kaninchen
    Klonalität
    • 8
    Polyklonal
    Konjugat
    • 5
    • 1
    • 1
    • 1
    Dieser CNST Antikörper ist unkonjugiert
    Applikation
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Verwendungszweck
    Rabbit polyclonal antibody raised against partial recombinant Cnst.
    Sequenz
    MAPEERRDSE DRVSKETEDY LHSLLERCLK DAEDSLSYED IQDDDSDLLQ DLSPEEASYS LQEDLPPDES TLSLDDLAKK IEIAEAIPAE GLVSILKKRN DTVGSHPAQM QQKPAKRRVR FQEID
    Spezifität
    Specific to recombinant protein GX2268. This antibody detects mC1orf71 protein.
    Kreuzreaktivität
    Maus
    Immunogen
    Recombinant GST fusion protein corresponding to 125 amino acids of mouse Cnst.
  • Applikationshinweise
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #102674 (Western Blotting)
    'Independent Validation' Siegel
    by
    Christoffels Lab, Heart Failure Research Center, Academisch Medisch Centrum Amsterdam
    No.
    #102674
    Datum
    14.05.2018
    Antigen
    CNST
    Chargennummer
    BIAD001
    Validierte Anwendung
    Western Blotting
    Positivkontrolle
    Whole E13.5 wildtype mouse embryo protein lysate
    Negativkontrolle
    Whole E13.5 mouse embryo protein lysates homozygous for a null Cnst allele. This mouse line was generated using CRISPR/Cas9.
    Bewertung

    Passed. ABIN5575708 specifically recognizes Cnst in whole embryo lysates.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN5575708
    Sekundärantikörper
    anti-rabbit HRP-conjugated antibody (GE Healthcare, NA934V, lot 9804671)
    Full Protocol
    • Isolate E13.5 mouse embryos in cold PBS and snap freeze with liquid nitrogen.
    • Lyse each individual embryo in 300µl of cold RIPA buffer.
    • Determine total protein content of the lysates using Pierce™ BCA Protein Assay Kit (Thermo Scientific, 23227, lot NA168207).
    • Denature 5µg of total protein for 5min at 95°C in 20µl 2x Laemmli SDS buffer and subsequently separate them on a 10% denaturing acrylamide gel in a BIO-RADMini-PROTEAN 3 chamber for 1h at 180V.
    • Transfer proteins onto PVDF membrane (Immobilon-P, Millipore) with a Western blotting system for 45min at 25V.
    • Block the membrane with 5% Milk in TBST as blocking buffer for 1-2h at RT.
    • Incubation with primary
      • rabbit anti-Consortin antibody (antibodies-online, ABIN5575708, lot BIAD001) diluted 1:2000 in blocking buffer 5% milk ON at 4°C.
      • mouse anti-GAPDH antibody (Santa Cruz, sc-365062, lot I0916) diluted 1:5000 in blocking buffer ON at 4°C.
    • Wash membrane 5x for 15min with TBST.
    • Incubation with secondary
      • anti-rabbit HRP-conjugated antibody (GE Healthcare, NA934V, lot 9804671) diluted 1:2000 blocking buffer for 1-2h at RT.
      • anti-mouse HRP-conjugated antibody (GE Healthcare, NA931V, lot 9800151) diluted 1:2000 in blocking buffer for 1-2h at RT.
    • Wash membrane 5x for 15min with TBST.
    • Reveal protein bands using ECL detection kit (GE Healthcare) on an ImageQuant LAS 4000 phosphoimaging unit (GE Healthcare).
    Anmerkungen

    The Consortin antibody ABIN5575708 reveals a protein of the expected molecular weight of antigen in lysates of whole E13.5 embryos. The protein bands is only visible in the positive but not the negative controls.

  • Format
    Liquid
    Buffer
    In PBS (50 % glycerol, 0.02 % sodium azide)
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Target
    CNST (Consortin, Connexin Sorting Protein (CNST))
    Andere Bezeichnung
    9630058J23Rik (CNST Produkte)
    Synonyme
    C1orf71 antikoerper, PPP1R64 antikoerper, 9630058J23Rik antikoerper, consortin, connexin sorting protein antikoerper, CNST antikoerper, Cnst antikoerper
    Hintergrund
    Full Gene Name: RIKEN cDNA 9630058J23 gene
    Synonyms: MGC31547
    Gen-ID
    226744
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