FBXO32 Antikörper (Internal Region, Isoform 1)

Produktnummer ABIN375094

Produktdetails anti-FBXO32 Antikörper

Target: FBXO32 (F-Box Protein 32 (FBXO32))

Bindungsspezifität: Internal Region, Isoform 1

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FBXO32 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: This antibody is expected to recognize reported isoform 1 (FBXO32).

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Bovine and Porcine.
Species reactivity (tested):Human, Mouse

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence: C-NSKTKTQYFHQEK from the internal region of F-box only protein 32 isoform 1 / FBXO32

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/32000. Western Blot: 0.1-0.3 μg/mL. Approx.40 kDa band observed in Human and Mouse SkeletalMuscle lysates (calculated MW of 41.0 kDa according to Human NP_478136.1 and of41.0 kDa according to Mouse NP_080622.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu FBXO32

Target: FBXO32 (F-Box Protein 32 (FBXO32))

Andere Bezeichnung: FBXO32 (FBXO32 Produkte)

Synonyme: MGC56479 antikoerper, zgc:56479 antikoerper, Fbx32 antikoerper, MAFbx antikoerper, 4833442G10Rik antikoerper, AI430017 antikoerper, ATROGIN1 antikoerper, Gm20361 antikoerper, Atrogin1 antikoerper, F-box protein 32 antikoerper, fbxo32 antikoerper, FBXO32 antikoerper, Fbxo32 antikoerper

Hintergrund: Fbx32 is an E3 ubiquitin ligase that initiates ATP dependent ubiquitin-mediated proteolysis and promotes muscle atrophy. It is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. It is also thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. Fbx32 interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD.Synonyms: Atrogin-1, F-box only protein 32, FBXO32, MAFbx, Muscle atrophy F-box protein

Gen-ID: 114907, 9606

UniProt: Q969P5