MRPL3 Antikörper (C-Term)

Produktnummer ABIN374279

Produktdetails anti-MRPL3 Antikörper

Target: MRPL3 (Mitochondrial Ribosomal Protein L3 (MRPL3))

Bindungsspezifität: C-Term

Reaktivität: Human, Rind (Kuh), Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MRPL3 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Sequenz: NVCQPGAPSI TFA

Spezifität: This antibody detects MRPL3 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Pig, CowSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_009139.1 Genename: MRPL3

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 35 kDa band observed in lysates of cell line HepG2(calculated MW of 38.5 kDa according to NP_009139.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu MRPL3

Target: MRPL3 (Mitochondrial Ribosomal Protein L3 (MRPL3))

Andere Bezeichnung: MRPL3 (MRPL3 Produkte)

Synonyme: COXPD9 antikoerper, MRL3 antikoerper, RPML3 antikoerper, GB13776 antikoerper, 2010320L16Rik antikoerper, 5930422H18Rik antikoerper, AA407889 antikoerper, mrpl3 antikoerper, fj61d01 antikoerper, wu:fj61d01 antikoerper, zgc:66423 antikoerper, mitochondrial ribosomal protein L3 antikoerper, 39S ribosomal protein L3, mitochondrial antikoerper, mitochondrial 54S ribosomal protein YmL3 antikoerper, mitochondrial ribosomal protein L3 S homeolog antikoerper, MRPL3 antikoerper, LOC552676 antikoerper, Mrpl3 antikoerper, mrpl3.S antikoerper, mrpl3 antikoerper

Hintergrund: Mammalian mitochondrial ribosomal proteins (MRPs) are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This antibody targets a 39S subunit protein that belongs to the L3P ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 13q.Synonyms: 39S ribosomal protein L3, L3mt, MRL3, MRP-L3, RPML3, mitochondrial, mitochondrial 60S ribosomal protein L3

Gen-ID: 11222

NCBI Accession: NP_009139

UniProt: P09001