Apolipoprotein M Antikörper (C-Term)

Produktnummer ABIN452682

Produktdetails anti-Apolipoprotein M Antikörper

Target: Apolipoprotein M (APOM)

Bindungsspezifität: C-Term

Reaktivität: Human, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Apolipoprotein M Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody recognizes Apolipoprotein M (Apo M).

Kreuzreaktivität (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PRNQEACELSNN, from the C Terminus of the protein sequence according to NP_061974.2.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Antibody Detection Limit Dilution: 1/8000. Western Blot: Preliminary experiments gave an approx 50 kDa band in Human Kidney andLiver lysates after 0.2 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 21.0 kDaaccording to NP_061974.2. We do note that ApoM is a glycoprotein which makes a highersize band to be expected. The 50 kDa band was successfully blocked by incubation withthe immunizing peptide. Immunohistochemistry: 3-6 μg/mL. In paraffin embedded Human Liver shows strong stain of cytoplasm in select hepatocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu Apolipoprotein M

Target: Apolipoprotein M (APOM)

Andere Bezeichnung: Apolipoprotein M (Apo M) (APOM Produkte)

Synonyme: G3a antikoerper, HSPC336 antikoerper, NG20 antikoerper, apo-M antikoerper, 1190010O19Rik antikoerper, APOM antikoerper, fb62d12 antikoerper, wu:fb62d12 antikoerper, g3a antikoerper, ng20 antikoerper, hspc336 antikoerper, mKIAA4033 antikoerper, apolipoprotein M antikoerper, apolipoprotein M S homeolog antikoerper, myeloperoxidase antikoerper, APOM antikoerper, Apom antikoerper, apom.S antikoerper, apom antikoerper, Mpo antikoerper

Hintergrund: Apolipoprotein M is a member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. It is positively related to leptin. It is mainly expressed in liver and kidney. Apolipoprotein M is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. It is important for the formation of prebeta-HDL and cholesterol efflux to HDL, and thereby inhibits formation of atherosclerotic lesions.Synonyms: Apo-M, ApoM, HSPC336, NG20, Protein G3A

Gen-ID: 55937

NCBI Accession: NP_061974

UniProt: O95445