NMDAR2A Antikörper (C-Term)

Produktnummer ABIN372679

Produktdetails anti-NMDAR2A Antikörper

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Bindungsspezifität: C-Term

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NMDAR2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This antibody recognizes the ~180k NR2A subunit of the NMDA Receptor in Western Blots of most brain regions. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse and Rat.

Aufreinigung: Affinity Chromatography.

Immunogen: Fusion protein from the C-terminus of the NR2A subunit of Rat NMDA Receptor.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 1/1000. Immunohistochemistry on Frozen Sections: 1/1000-1/2000. Immunoprecipitation: 3 μL per 200 μg lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 50 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.

Buffer: 5 mM Ammonium Bicarbonate.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Referenzen für anti-NMDAR2A Antikörper (ABIN372679)

Bostrom, Majaess, Morch, White, Eadie, Christie: "Rescue of NMDAR-dependent synaptic plasticity in Fmr1 knock-out mice." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 25, Issue 1, pp. 271-9, (2014) (PubMed).

Pozniak, Sengupta Ghosh, Gogineni, Hanson, Lee, Larson, Solanoy, Bustos, Li, Ngu, Jubb, Ayalon, Wu, Scearce-Levie, Zhou, Weimer, Kirkpatrick, Lewcock: "Dual leucine zipper kinase is required for excitotoxicity-induced neuronal degeneration." in: The Journal of experimental medicine, Vol. 210, Issue 12, pp. 2553-67, (2013) (PubMed).

Hicklin, Wu, Radcliffe, Freund, Goebel-Goody, Correa, Proctor, Lombroso, Browning: "Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 16, pp. 6650-5, (2011) (PubMed).

Norris, Strickland: "Modulation of NR2B-regulated contextual fear in the hippocampus by the tissue plasminogen activator system." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 33, pp. 13473-8, (2007) (PubMed).

Wenthold, Prybylowski, Standley, Sans, Petralia: "Trafficking of NMDA receptors." in: Annual review of pharmacology and toxicology, Vol. 43, pp. 335-58, (2003) (PubMed).

Alvestad, Grosshans, Coultrap, Nakazawa, Yamamoto, Browning: "Tyrosine dephosphorylation and ethanol inhibition of N-Methyl-D-aspartate receptor function." in: The Journal of biological chemistry, Vol. 278, Issue 13, pp. 11020-5, (2003) (PubMed).

Details zu NMDAR2A

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Abstract: GRIN2A Produkte

Synonyme: NMDAR2A antikoerper, nr2a antikoerper, GRIN2A antikoerper, EPND antikoerper, GluN2A antikoerper, NR2A antikoerper, glutamate ionotropic receptor NMDA type subunit 2A antikoerper, glutamate receptor, ionotropic, N-methyl D-aspartate 2A L homeolog antikoerper, glutamate receptor ionotropic, NMDA 2A antikoerper, glutamate receptor, ionotropic, NMDA2A (epsilon 1) antikoerper, GRIN2A antikoerper, grin2a.L antikoerper, LOC100438553 antikoerper, Grin2a antikoerper

Hintergrund: The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.Synonyms: GRIN2A, Glutamate [NMDA] Receptor subunit epsilon-1, NMDAR2A, NR2A

Gen-ID: 2903

NCBI Accession: NP_000824

UniProt: Q12879

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity