PMS2 Antikörper (AA 58-81)

Produktnummer ABIN400795

Produktdetails anti-PMS2 Antikörper

Target: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Bindungsspezifität: AA 58-81

Reaktivität: Human, Maus, Ratte, Hamster, Schimpanse

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PMS2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Spezifität: This antibody is directed against PMS2-134 and reacts with full length version of PMS2. The epitope was putatively mapped to amino acids 58-81 of human PMS2. Expect a band approximately 96 kDa in size corresponding to human PMS2 by western blotting in most cell lines and tissues as PMS2 is ubiquitously expressed.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Chimpanzee (100 %)Species reactivity (tested):Human, Hamster

Produktmerkmale: Synonyms: PMSL2, Mismatch repair endonuclease PMS2, PMS1 protein homolog 2

Aufreinigung: Protein chromatography

Immunogen: Recombinant human PMS2 corresponding to the first 133 amino acid residues of theprotein

Klon: 349-29-5-2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 5,000 - 1: 20,000. Western blot: 1: 500 - 1: 2,000. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.38 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 a buffer and 0.09 % (w/v) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C forlonger. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu PMS2

Target: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Andere Bezeichnung: PMS2 (PMS2 Produkte)

Synonyme: HNPCC4 antikoerper, PMS2CL antikoerper, PMSL2 antikoerper, AW555130 antikoerper, Pmsl2 antikoerper, PMS1 homolog 2, mismatch repair system component antikoerper, PMS1 homolog2, mismatch repair system component antikoerper, mismatch repair endonuclease PMS2 antikoerper, PMS2 antikoerper, Pms2 antikoerper, LOC463257 antikoerper, LOC107984056 antikoerper

Hintergrund: PMS2 is a highly conserved nuclear protein involved in mismatch repair during DNA replication and has been identified to be composed as a heterodimer of PMS2 and MLH1. PMS is part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4), Turcot syndrome (an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas) and supratentorial primitive neuroectodermal tumors with cafe-au-lait spots (SNTCL). The human PMS2 gene encodes an 862 aa, 96 kDa polypeptide.Synonyms: DNA mismatch repair protein PMS2, Mismatch repair endonuclease PMS2, PMS1 protein homolog 2, PMSL2

Gen-ID: 5395

UniProt: P54278

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response