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ODF2 Antikörper

ODF2 Reaktivität: Human, Ratte, Maus WB, ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN5548695
  • Target Alle ODF2 Antikörper anzeigen
    ODF2 (Outer Dense Fiber of Sperm Tails 2 (ODF2))
    Reaktivität
    • 31
    • 21
    • 14
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Human, Ratte, Maus
    Wirt
    • 44
    • 2
    • 1
    Kaninchen
    Klonalität
    • 44
    • 3
    Polyklonal
    Konjugat
    • 19
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ODF2 Antikörper ist unkonjugiert
    Applikation
    • 32
    • 13
    • 13
    • 12
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Aufreinigung
    Affinity purification
    Immunogen
    Recombinant protein of human ODF2
    Isotyp
    IgG
  • Applikationshinweise
    WB 1:500-1:2000, IHC 1:25-1:100
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #100023 (Western Blotting)
    'Independent Validation' Siegel
    by
    Georg-August-University of Göttingen, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Developmental Biology
    No.
    #100023
    Datum
    03.05.2016
    Antigen
    Outer Dense Fiber of Sperm Tails 2 (ODF2)
    Chargennummer
    Validierte Anwendung
    Western Blotting
    Positivkontrolle
    recombinant ODF2-His, bacterially expressed; NIH3T3 total cell lysate
    Negativkontrolle
    Bewertung
    Passed, regarding sensitivity and specificity. The antibody detects bacterially expressed ODF2 (see 2)) and the endogenous protein (see 3)) with only moderate background staining in WB.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN2430582
    Sekundärantikörper
    anti rabbit IgG (H+L), HRP-linked (Jackson Immuno Research, Code number: 111-035-003, Lot number: 123450)
    Full Protocol
    • Proteins separated on 10% SDS-PAGE (Laemmli 1970), blotted to Protran (GE Healthcare No. 10600004, 0.2 µm) (Towbin et al., 1979). Blocking of membrane in 5% skim milk in TBST (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1% Tween 20) for 30 min at room temperature.
    • Incubation with first antibody in 5% skim milk in TBST at 4°C overnight.
    • Washing in TBST for 30 minutes at room temperature.
    • Incubation with secondary antibody in 5% skim milk in TBST for 45 minutes at room temperature. Washing in TBST for 30-45 minutes at room temperature.
    • Secondary antibody: anti rabbit IgG (H+L), HRP-linked (Jackson Immuno Research, Code number: 111-035-003, Lot number: 123450). Dilution: 1:10,000.
    • Chemiluminescence detection (Western Lightning Ultra, Lot 215-15091, Perkin Elmer) and image capture via BioRad Chemdoc or X-ray films.
    Anmerkungen
  • Validierung #100024 (Immunocytochemistry)
    'Independent Validation' Siegel
    by
    Georg-August-University of Göttingen, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Developmental Biology
    No.
    #100024
    Datum
    03.05.2016
    Antigen
    Outer Dense Fiber of Sperm Tails 2 (ODF2)
    Chargennummer
    Validierte Anwendung
    Immunocytochemistry
    Positivkontrolle
    mouse anti-γ-tubulin (Sigma-Aldrich, clone GTU-88, T6557, lot number 072M4808)
    Negativkontrolle
    Bewertung
    Passed, the antibody detects centrosomal ODF2 in immunocytological preparations with only very low background staining (the green dots are mostly the correct location of ODF2 as they overlap with γ-tubulin spots (in red)).
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN2430582
    Sekundärantikörper
    MFP488 goat anti-rabbit IgG (H+L) (MoBiTec, #MFP-A1008)
    Full Protocol
    • NIH3T3 cells (ATCC) were grown on cover slips in DMEM, 10% fetal bovine serum, 5% penicillin/streptomycin (all Gibco) at 37°C in 5% CO2.
    • Cells were fixed in ice-cold methanol for 20 min at -20°C.
    • Unspecific binding sites were blocked in PBST (phosphate buffered saline (PBS) containing 0.15% bovine serum albumin, 0.1% Tween-20) for 1 hr.
    • Cells were incubated with the primary antibodies at 4°C overnight.
    • Cells were washed in TBST (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1% Tween 20) for 15 min.
    • Cells were incubated with secondary antibodies and DAPI for 1h at 37°C.
    • Cells were washed in TBST for 15 min.
    • Cells were embedded in Dako Fluorescent Mounting Medium (Dako).
    • As positive control for centrosomal decoration anti ?-tubulin (Sigma-Aldrich, clone GTU-88, T6557, lot number 072M4808; 1:100) was used. anti ?-tubulin was detected with MFP590 goat anti-mouse IgG (H+L) (MoBiTec, #MFP-A1032; 1:300).
    • Anti ODF2-antibody was detected with MFP488 goat anti-rabbit IgG (H+L) (MoBiTec, #MFP-A1008; 1:300).
    • DNA was counterstained with DAPI.
    • Images were taken by confocal microscopy (LSM 510, Zeiss), and processed using Adobe Photoshop 5.0.
    Anmerkungen
  • Format
    Liquid
    Konzentration
    0.3 mg/mL
    Buffer
    PBS with 0.05 % sodium azide and 50 % glycerol, PH7.4
    Konservierungsmittel
    Sodium azide
    Handhabung
    Avoid freeze / thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at -20°C. Avoid freeze / thaw cycles.
  • Rahimi, Cai, Kılıҫ, Kazerouni, Tapia Contreras, Hoyer-Fender: "Expression of α-Tubulin Acetyltransferase 1 and Tubulin Acetylation as Selective Forces in Cell Competition." in: Cells, Vol. 10, Issue 2, (2021) (PubMed).

    Tapia Contreras, Hoyer-Fender: "CCDC42 Localizes to Manchette, HTCA and Tail and Interacts With ODF1 and ODF2 in the Formation of the Male Germ Cell Cytoskeleton." in: Frontiers in cell and developmental biology, Vol. 7, pp. 151 (PubMed).

  • Target
    ODF2 (Outer Dense Fiber of Sperm Tails 2 (ODF2))
    Andere Bezeichnung
    ODF2 (ODF2 Produkte)
    Synonyme
    odf84 antikoerper, odf2/1 antikoerper, odf2/2 antikoerper, DKFZp469J0214 antikoerper, CT134 antikoerper, ODF2/1 antikoerper, ODF2/2 antikoerper, ODF84 antikoerper, si:dkey-228b2.4 antikoerper, AI848335 antikoerper, MMTEST29 antikoerper, outer dense fiber of sperm tails 2 antikoerper, outer dense fiber of sperm tails 2a antikoerper, outer dense fiber of sperm tails 2 L homeolog antikoerper, ODF2 antikoerper, odf2 antikoerper, odf2a antikoerper, odf2.L antikoerper, Odf2 antikoerper
    Hintergrund
    The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants.
    Molekulargewicht
    Calculated MW: 95 kDa
    Pathways
    M Phase
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