Dieser Topoisomerase II alpha Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Aufreinigung
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This TOP2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1167-1196 amino acids from the C-terminal region of human TOP2A.
TOP2A
Reaktivität: Human
WB, ELISA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
0.35 mg/mL
Buffer
Supplied in PBS with 0.09 % (W/V) sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
Topoisomerase II alpha (TOP2A)
(Topoisomerase (DNA) II alpha 170kDa (TOP2A))
TOP2 antikoerper, TP2A antikoerper, TOP2A antikoerper, Top-2 antikoerper, TOP-2 antikoerper, F12B17.110 antikoerper, F12B17_110 antikoerper, thimet metalloendopeptidase 2 antikoerper, fc08c07 antikoerper, wu:fc08c07 antikoerper, DNA topoisomerase II alpha antikoerper, DNA topoisomerase II, alpha isozyme antikoerper, topoisomerase (DNA) II alpha antikoerper, Zincin-like metalloproteases family protein antikoerper, TOP2A antikoerper, Bm1_08705 antikoerper, Top2a antikoerper, AT5G10540 antikoerper, top2a antikoerper
Hintergrund
TOP2A encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA.