CD31 Antikörper (C-Term)

Produktnummer ABIN5518694

Produktdetails anti-CD31 Antikörper

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Bindungsspezifität: AA 717-732, C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD31 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Rabbit IgG polyclonal antibody for Platelet endothelial cell adhesion molecule(PECAM1) detection. Tested with WB, IHC-P, IHC-F in Human.

Sequenz: RKAVPDAVES RYSRTE

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Platelet endothelial cell adhesion molecule(PECAM1) detection. Tested with WB, IHC-P, IHC-F in Human.
Gene Name: platelet/endothelial cell adhesion molecule 1
Protein Name: Platelet endothelial cell adhesion molecule

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CD31(717-732aa RKAVPDAVESRYSRTE).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for PECAM1 is approximately 5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-CD31 Antikörper (ABIN5518694)

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Fan, Qu, Chu, Gao, Gao, Chen, Tian: "MicroRNA-210 promotes angiogenesis in acute myocardial infarction." in: Molecular medicine reports, Vol. 17, Issue 4, pp. 5658-5665, (2018) (PubMed).

Atala: "Re: Treatment of Diabetes Mellitus-Induced Erectile Dysfunction Using Endothelial Progenitor Cells Genetically Modified with Human Telomerase Reverse Transcriptase." in: The Journal of urology, Vol. 197, Issue 2, pp. 494, (2017) (PubMed).

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Chen, Yu, Gumireddy, Li, Yao, Gao, Chen, Hao, Wang, Huang, Xu, Ye: "ZBRK1, a novel tumor suppressor, activates VHL gene transcription through formation of a complex with VHL and p300 in renal cancer." in: Oncotarget, Vol. 6, Issue 9, pp. 6959-76, (2016) (PubMed).

Wang, Yang, Tang, Long, Huang: "Wound Dressing Model of Human Umbilical Cord Mesenchymal Stem Cells-Alginates Complex Promotes Skin Wound Healing by Paracrine Signaling." in: Stem cells international, Vol. 2016, pp. 3269267, (2016) (PubMed).

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Zeng, Zhao, Ouyang, Zhao, Zhang, Chen, Yu, Lei: "Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium." in: Oncology letters, Vol. 12, Issue 2, pp. 815-824, (2016) (PubMed).

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Details zu CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Andere Bezeichnung: PECAM1 (PECAM1 Produkte)

Synonyme: CD31 antikoerper, CD31/EndoCAM antikoerper, GPIIA' antikoerper, PECA1 antikoerper, PECAM-1 antikoerper, endoCAM antikoerper, C85791 antikoerper, Cd31 antikoerper, Pecam antikoerper, platelet/endothelial cell adhesion molecule 1 antikoerper, platelet and endothelial cell adhesion molecule 1 antikoerper, pecam1 antikoerper, PECAM1 antikoerper, Pecam1 antikoerper

Hintergrund: PECAM-1(Platelet endothelial cell adhesion molecule), also known as cluster of differentiation 31(CD31) is a protein that in human is encoded by the PECAM1 gene found on chromosome 17. PECAM1 is a member of the immunoglobulin(Ig) superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and particular T-cell subsets. Using a PCR-based analysis of somatic cell hybrids, Gumina et al.(1996) mapped PECAM1 to chromosome 17 in the region 17q23-qter. Several adhesion molecules expressed on platelets and endothelium also localized to 17q. Xie and Muller(1996) mapped the Pecam1 gene to mouse chromosome 6, region F3-G1, by fluorescence in situ hybridization. PECAM-1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions, and PECAM-1 plays a key role in removing aged neutrophils from the body.

Synonyms: Adhesion molecule antibody|CD31 antibody|CD31 antigen antibody|CD31 EndoCAM antibody|Endocam antibody|FLJ34100 antibody|FLJ58394 antibody|GPIIA antibody|GPIIA' antibody|PECA1 antibody|PECA1_HUMAN antibody|Pecam 1 antibody|PECAM 1 CD31 EndoCAM antibody|PECAM antibody|PECAM-1 antibody|Pecam1 antibody|Platelet endothelial cell adhesion molecule antibody|Platelet/endothelial cell adhesion molecule 1 antibody|Platelet/endothelial cell adhesion molecule antibody

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization