ELISA (1:1000) Dot Blot (1:500) The optimal working dilution should be determined by the end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
In PBS (0.09 % sodium azide)
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Nilsen, Slagsvold, Skjerpen, Brech, Stenmark, Olsnes: "Peroxisomal targeting as a tool for assaying potein-protein interactions in the living cell: cytokine-independent survival kinase (CISK) binds PDK-1 in vivo in a phosphorylation-dependent manner." in: The Journal of biological chemistry, Vol. 279, Issue 6, pp. 4794-801, (2004) (PubMed).
Collins, Deak, Arthur, Armit, Alessi: "In vivo role of the PIF-binding docking site of PDK1 defined by knock-in mutation." in: The EMBO journal, Vol. 22, Issue 16, pp. 4202-11, (2003) (PubMed).
Egawa, Maegawa, Shi, Nakamura, Obata, Yoshizaki, Morino, Shimizu, Nishio, Suzuki, Kashiwagi et al.: "Membrane localization of 3-phosphoinositide-dependent protein kinase-1 stimulates activities of Akt and atypical protein kinase C but does not stimulate glucose transport and glycogen synthesis in ..." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38863-9, (2002) (PubMed).
Target
PDPK1
(3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))