ELISA (1:1000) Western Blot (1:50-100) Immunohistochemistry (1:10-50) The optimal working dilution should be determined by the end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
In PBS (0.09 % sodium azide)
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Nogueira da Costa, Keen, Wild, Findlay: "An analysis of the phosphoproteome of immune cell lines exposed to the immunomodulatory mycotoxin deoxynivalenol." in: Biochimica et biophysica acta, Vol. 1814, Issue 7, pp. 850-7, (2011) (PubMed).
Kondo, Hirayama, Sugito, Shono, Tanaka, Kitamura: "Coupling of Grb2 to Gab1 mediates hepatocyte growth factor-induced high intensity ERK signal required for inhibition of HepG2 hepatoma cell proliferation." in: The Journal of biological chemistry, Vol. 283, Issue 3, pp. 1428-36, (2008) (PubMed).
Martínez, García-Domínguez, Domingo, Oliva, Zarich, Sánchez, Gutiérrez-Eisman, Llopis, Rojas: "Sprouty2 binds Grb2 at two different proline-rich regions, and the mechanism of ERK inhibition is independent of this interaction." in: Cellular signalling, Vol. 19, Issue 11, pp. 2277-85, (2007) (PubMed).
Morimatsu, Takagi, Ota, Iwamoto, Yanagida, Sako: "Multiple-state reactions between the epidermal growth factor receptor and Grb2 as observed by using single-molecule analysis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 46, pp. 18013-8, (2007) (PubMed).
Target
GRB2
(Growth Factor Receptor-Bound Protein 2 (GRB2))