Immunofluorescence (1:10-50) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
In PBS (0.09 % sodium azide).
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store at 4°C for up to 2 weeks. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Márquez, Smith, Petoukhov, Lo Surdo, Mattsson, Knekt, Westlund, Scheffzek, Saraste, Svergun: "Conformation of full-length Bruton tyrosine kinase (Btk) from synchrotron X-ray solution scattering." in: The EMBO journal, Vol. 22, Issue 18, pp. 4616-24, (2003) (PubMed).
Jefferies, Doyle, Brunner, Dunne, Brint, Wietek, Walch, Wirth, ONeill: "Bruton's tyrosine kinase is a Toll/interleukin-1 receptor domain-binding protein that participates in nuclear factor kappaB activation by Toll-like receptor 4." in: The Journal of biological chemistry, Vol. 278, Issue 28, pp. 26258-64, (2003) (PubMed).
Horwood, Mahon, McDaid, Campbell, Mano, Brennan, Webster, Foxwell: "Bruton's tyrosine kinase is required for lipopolysaccharide-induced tumor necrosis factor alpha production." in: The Journal of experimental medicine, Vol. 197, Issue 12, pp. 1603-11, (2003) (PubMed).