Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Verwendungszweck
Rabbit polyclonal antibody raised against full length recombinant S100A1/S100B.
Spezifität
A 98 % homology is noted for S100 alpha chain from primate sources. Mouse, rat and dog show 94 % homology with the bovine S100 alpha sequence. Expect cross reactivity with S100 from most mammalian sources. Homologies for the S100 beta chain are similar.
Kreuzreaktivität
Rind (Kuh), Hund, Maus, Ratte
Produktmerkmale
Antibody Reactive Against Recombinant Protein.
Immunogen
Recombinant protein corresponding to full length bovine S100A1/S100B.
S100A/B
Reaktivität: Human
WB, ELISA
Wirt: Maus
Monoclonal
1-B-657
HRP
Applikationshinweise
ELISA (1:5000-1:25000) Western Blot (1:500-1:3000) Immunohistochemistry (1:200-1:2000) The optimal working dilution should be determined by the end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store at 4°C. For long term storage store at -20°C. Aliquot after reconstitution to avoid repeated freezing and thawing.
Charron, Doudnikoff, Canron, Li, Véga, Marais, Baufreton, Vital, Oliet, Bezard: "Astrocytosis in parkinsonism: considering tripartite striatal synapses in physiopathology?" in: Frontiers in aging neuroscience, Vol. 6, pp. 258, (2014) (PubMed).
Charron, Doudnikoff, Laux, Berthet, Porras, Canron, Barroso-Chinea, Li, Qin, Nosten-Bertrand, Giros, Delalande, Van Dorsselaer, Vital, Goumon, Bezard: "Endogenous morphine-like compound immunoreactivity increases in parkinsonism." in: Brain : a journal of neurology, Vol. 134, Issue Pt 8, pp. 2321-38, (2011) (PubMed).