E2F1 Antikörper (pSer364)

Produktnummer ABIN538143

Produktdetails anti-E2F1 Antikörper

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Bindungsspezifität: pSer364

Reaktivität: Human, Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit polyclonal antibody raised against synthetic phosphopeptide of E2F1.

Spezifität: Reactivity occurs against human E2F-1 pS364 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human E2F-1 is minimal by ELISA. This antibody does not cross-react with E2F-1 phosphorylated at other sites.

Kreuzreaktivität: Schimpanse, Human

Produktmerkmale: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S364 of human E2F1.

Anwendungsinformationen

Applikationshinweise: ELISA (1:20000-1:100000)
Western Blot 1:250-1:2000
Immunohistochemistry (2-20 μg/mL)
The optimal working dilution should be determined by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Referenzen für anti-E2F1 Antikörper (ABIN538143)

Wang, Hou, Mohapatra, Ma, Cress, Pledger, Chen: "Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12339-43, (2005) (PubMed).

Joshi, Ordonez-Ercan, Dasgupta, Chellappan: "Induction of human metallothionein 1G promoter by VEGF and heavy metals: differential involvement of E2F and metal transcription factors." in: Oncogene, Vol. 24, Issue 13, pp. 2204-17, (2005) (PubMed).

Sdek, Ying, Zheng, Margulis, Tang, Tian, Xiao: "The central acidic domain of MDM2 is critical in inhibition of retinoblastoma-mediated suppression of E2F and cell growth." in: The Journal of biological chemistry, Vol. 279, Issue 51, pp. 53317-22, (2004) (PubMed).

Details zu E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Andere Bezeichnung: E2F1 (E2F1 Produkte)

Synonyme: CG6376 antikoerper, DRTF1/E2F antikoerper, DmE2F-1 antikoerper, Dmel\\CG6376 antikoerper, Dp antikoerper, E(Sev-CycE)3A antikoerper, E(var)3-93E antikoerper, E(var)3-95E antikoerper, E(var)93E antikoerper, E2F antikoerper, E2F-1 antikoerper, E2F1 antikoerper, E2f1 antikoerper, Evar(3)164 antikoerper, dE2F antikoerper, dE2F1 antikoerper, dE2f antikoerper, dE2f1 antikoerper, de2f1 antikoerper, def21 antikoerper, drosE2F1 antikoerper, e2f antikoerper, e2f1 antikoerper, l(3)07172 antikoerper, l(3)j3B1 antikoerper, l(3)j3C2 antikoerper, l(3)rM729 antikoerper, RBAP1 antikoerper, RBBP3 antikoerper, RBP3 antikoerper, mKIAA4009 antikoerper, xE2F antikoerper, ATE2FB antikoerper, E2F TRANSCRIPTION FACTOR-1 E2F1 antikoerper, E2F transcription factor 1 antikoerper, E2FB antikoerper, E2FB TRANSCRIPTION FACTOR antikoerper, E2F transcription factor 1 antikoerper, E2F transcription factor 1 L homeolog antikoerper, E2f1 antikoerper, E2F1 antikoerper, e2f1.L antikoerper

Gen-ID: 1869

Pathways: p53 Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagie