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HSP70 Antikörper (Atto 390)

HSP70 Reaktivität: Human WB, IF, ICC, FACS Wirt: Maus Monoclonal 1H11 Atto 390
Produktnummer ABIN5066930
  • Target Alle HSP70 Antikörper anzeigen
    HSP70 (Heat Shock Protein 70 (HSP70))
    Reaktivität
    • 323
    • 199
    • 165
    • 71
    • 62
    • 53
    • 52
    • 52
    • 36
    • 33
    • 29
    • 29
    • 28
    • 23
    • 20
    • 18
    • 15
    • 13
    • 12
    • 12
    • 10
    • 10
    • 9
    • 9
    • 9
    • 9
    • 7
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 206
    • 150
    • 13
    • 11
    • 11
    Maus
    Klonalität
    • 228
    • 162
    Monoklonal
    Konjugat
    • 112
    • 31
    • 28
    • 26
    • 24
    • 20
    • 19
    • 18
    • 18
    • 18
    • 12
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Dieser HSP70 Antikörper ist konjugiert mit Atto 390
    Applikation
    • 374
    • 159
    • 142
    • 122
    • 122
    • 110
    • 101
    • 66
    • 62
    • 61
    • 41
    • 33
    • 22
    • 21
    • 21
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Spezifität
    Induced by heat shock.,Conformation-spec ab for mem-bound HSP70. Binds to cell surface HSP70 w/o permeabilization of cell mem in tumor cells. Does't bind to Intra-C HSP70 at low conc. X-reacts with HSC70. ~70 kDa in WB.
    Kreuzreaktivität
    Rind (Kuh), Human, Maus, Schwein, Ratte
    Aufreinigung
    Protein G Purified
    Immunogen
    Human native HSP70 protein
    Klon
    1H11
    Isotyp
    IgG1
    Top Product
    Discover our top product HSP70 Primärantikörper
  • Applikationshinweise
    • WB (1:1000)
    • ICC/IF (1:100)
    • FACS (1:250)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    A 1:250 dilution of ABIN5066930 was sufficient for detection of cell surface HSP70 in HCT116 cells using Fluorescence-activated cell sorting, applied in flow cytometry, with FITC as the fluorescent probe.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Conjugated antibodies should be stored at 4°C
  • Target
    HSP70 (Heat Shock Protein 70 (HSP70))
    Andere Bezeichnung
    HSP70 (HSP70 Produkte)
    Synonyme
    APG-2 antikoerper, HS24/P52 antikoerper, HSPH2 antikoerper, RY antikoerper, hsp70 antikoerper, hsp70RY antikoerper, CG31354 antikoerper, HSP70 antikoerper, Hsp70Bb antikoerper, hsp70B antikoerper, hsp70Bb-prime antikoerper, DmelCG5834 antikoerper, CG5834 antikoerper, HSPA1 antikoerper, HSP70B' antikoerper, HSPA6 antikoerper, ARABIDOPSIS HEAT SHOCK PROTEIN 70 antikoerper, ATHSP70 antikoerper, heat shock protein 70 antikoerper, LOC100305036 antikoerper, hsc70 antikoerper, Hsp70 antikoerper, Hsp70-1 antikoerper, Hsp70.1 antikoerper, hsp68 antikoerper, Hsp110 antikoerper, irp94 antikoerper, HSP70-2 antikoerper, HSPA1B antikoerper, HSPA2 antikoerper, hsp70-5 antikoerper, HSP70-1 antikoerper, HSP70.1 antikoerper, HSP70.2 antikoerper, heat shock protein family A (Hsp70) member 4 antikoerper, CG5834 gene product from transcript CG5834-RA antikoerper, heat shock protein 70 antikoerper, heat shock protein family A (Hsp70) member 6 antikoerper, heat shock 70kDa protein 2 antikoerper, heat shock 70 kD protein cognate antikoerper, Hsp70 family chaperone antikoerper, Heat shock protein 70 antikoerper, Heat shock protein 70, putative antikoerper, heat shock protein 1B antikoerper, heat shock protein family A member 4 antikoerper, heat shock 70kDa protein 1A antikoerper, heat shock protein 1 antikoerper, Heat Shock Protein antikoerper, heat shock cognate 70-kd protein antikoerper, Heat shock 70 kDa protein 1A antikoerper, HSPA4 antikoerper, Hsp70Bbb antikoerper, HSP70 antikoerper, HSPA6 antikoerper, HSPA2 antikoerper, PCC7424_2419 antikoerper, Isop_1041 antikoerper, CGB_C3390W antikoerper, Bacsa_1698 antikoerper, dnaK-B antikoerper, LOC100305036 antikoerper, Hspa1b antikoerper, Hspa4 antikoerper, HSPA1A antikoerper, hsp1 antikoerper, hsp-70 antikoerper, hsp70 antikoerper, LOC108348108 antikoerper
    Hintergrund
    HSP70 is a highly conserved protein that is ubiquitously expressed. It can be found in chloroplasts, eukaryotic cytosol, endoplasmic reticulum, and mitochondria, but also embedded in the cell membrane and in the extracellular space (1,2,3,4). Even though HSP70 is one of the most studied heat shock proteins, the export mechanism and method of membrane insertion are not fully understood. The majority of the proteins in the heat shock family lack a consensus signal for secretion via the ER-Golgi pathway (5). Researchers have found that HSP70 may be released from cells via exosomes or secretory vesicles (6). Although HSP70 is ubiquitously expressed, there is not much information about its presence on cell surface. The finding that HSP70 is localized on the cell surface of cancer cells but not normal cells suggests a conformational change of HSP70 in the lower pH environment characteristic of cancer cells (7). The presence of cell membrane embedded HSP70 has been found to increase the stability of cancer cells, thereby protecting tumors from environmental stress (8, 9). Anti-HSP70 antibody, clone 1H11 (Catalog No. SMC-249) is unique from other commercially available antibodies in that it can bind to the extracellular region of the cell membrane embedded HSP70 protein, allowing researchers to differentiate between membrane bound and intracellular HSP70 across cancer cells types.
    Gen-ID
    3303
    NCBI Accession
    NP_005336
    UniProt
    P0DMV8, P0DMV9
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