SCNN1A Antikörper (AA 46-68) (Biotin)

Produktnummer ABIN5066868

Produktdetails anti-SCNN1A Antikörper (Biotin)

Target: SCNN1A (Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A))

Bindungsspezifität: AA 46-68

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SCNN1A Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Spezifität: Detects ~85 kDa.

Kreuzreaktivität: Maus, Ratte

Aufreinigung: Protein G Purified

Immunogen: Synthetic peptide from the N-terminal of Rat ENaC alpha (aa. 46-68)

Klon: 2G4

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:150)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN5066868 was sufficient for detection of ENaC alpha in 15 μg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu SCNN1A

Target: SCNN1A (Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A))

Andere Bezeichnung: ENaC alpha (SCNN1A Produkte)

Synonyme: BESC2 antikoerper, ENaCa antikoerper, ENaCalpha antikoerper, SCNEA antikoerper, SCNN1 antikoerper, ENaC antikoerper, Scnn1 antikoerper, mENaC antikoerper, SCNN1A antikoerper, alphaxENaC antikoerper, besc2 antikoerper, enaca antikoerper, scnn1 antikoerper, ENAC antikoerper, sodium channel epithelial 1 alpha subunit antikoerper, sodium channel, nonvoltage-gated 1 alpha antikoerper, sodium channel, non voltage gated 1 alpha subunit L homeolog antikoerper, Scnn1a antikoerper, SCNN1A antikoerper, scnn1a.L antikoerper

Hintergrund: The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).

Gen-ID: 25122

NCBI Accession: NP_113736

UniProt: Q6IRJ1