ELISA. Western blot: 1: 500 - 1: 1000, a 22 kDa band should be detected. Immunohistochemistry on paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
PBS containing 0.02 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Target
CRADD
(CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))
cradd antikoerper, MGC89035 antikoerper, zgc:101717 antikoerper, CRADD antikoerper, DKFZp468A2219 antikoerper, LOC100226369 antikoerper, raidd antikoerper, MGC78788 antikoerper, MRT34 antikoerper, RAIDD antikoerper, CASP2 and RIPK1 domain containing adaptor with death domain antikoerper, CASP2 and RIPK1 domain containing adaptor with death, gene 1 antikoerper, CASP2 and RIPK1 domain containing adaptor with death, gene 2 S homeolog antikoerper, CRADD antikoerper, cradd.1 antikoerper, cradd antikoerper, cradd.2.S antikoerper, Cradd antikoerper
Hintergrund
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain (DD)-containing receptors, TNFR1 and Fas. The death signals are transduced by a group of DD-containing adapter molecules. A novel cell death adapter was recently identified by two independent groups and designated RAIDD (RIP-associated ICH-1/CED-3-homologous protein with DD) and CRADD (caspase and RIP adapter with DD)1,RAIDD contains a DD and a CARD (for caspase recruitment domain) which interact with RIP and caspase, respectively, to transduce death signals. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR-1.Synonyms: CRADD, Caspase and RIP adapter with death domain, Death domain-containing protein CRADD, RIP-associated protein with a death domain