CPI-17 Antikörper

Produktnummer ABIN498637

Produktdetails anti-CPI-17 Antikörper

Target: CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CPI-17 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of CPI-17 protein. (region surrounding Lys32)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CPI-17

Target: CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))

Andere Bezeichnung: PPP1R14A (PPP1R14A Produkte)

Synonyme: MGC81874 antikoerper, cpi-17 antikoerper, ppp1inl antikoerper, CPI-17 antikoerper, im:7147597 antikoerper, ppp1r14a antikoerper, zgc:110700 antikoerper, PPP1R14A antikoerper, CPI17 antikoerper, PPP1INL antikoerper, Cpi17 antikoerper, 1110001M11Rik antikoerper, protein phosphatase 1 regulatory inhibitor subunit 14A L homeolog antikoerper, protein phosphatase 1 regulatory inhibitor subunit 14A antikoerper, protein phosphatase 1, regulatory (inhibitor) subunit 14Aa antikoerper, protein phosphatase 1, regulatory (inhibitor) subunit 14A antikoerper, ppp1r14a.L antikoerper, ppp1r14a antikoerper, ppp1r14aa antikoerper, PPP1R14A antikoerper, Ppp1r14a antikoerper

Hintergrund: CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. CPI-17 was originally identified as a 17 kDa PKC-potentiated inhibitory protein of protein phosphatase-1, which is dominantly expressed in smooth muscle. Phosphorylation at Threonine 38, in vitro, by PKC or Rho-kinase enhances the inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and might be involved in the calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid. CPI-17 is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase, M110 kinase.Synonyms: 17 kDa PKC-potentiated inhibitory protein of PP1, CPI-17, CPI17, PPP1INL, Protein phosphatase 1 regulatory subunit 14A

Molekulargewicht: approx. 22 kDa

Gen-ID: 94274

NCBI Accession: NP_150281

UniProt: Q96A00