Rekombinanter Kaninchen anti-Human IgG4 Antikörper
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- Target Alle IgG4 Produkte
- IgG4
- Antikörpertyp
- Recombinant Antibody
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Monoklonal
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- Produktmerkmale
- This recombinant Human IgG4 antibody reacts to the heavy chain of hIgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.
- Aufreinigung
- Protein A purified from animal origin-free supernatant
- Immunogen
- Peptide corresponding to the hinge region of human IgG4 was used as the immunogen for this recombinant Human IgG4 antibody.
- Klon
- RM120
- Isotyp
- IgG
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- Applikationshinweise
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The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. ELISA: 50 ng/well - 200 ng/well (Capture), 0.05-0.2 μg/mL (Detection),Western Blot: 0.5-2 μg/mL,Immunocytochemistry: 1-10 μg/mL,Immunohistochemistry: 1-10 μg/mL (1)
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store the recombinant Human IgG4 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
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- Target
- IgG4
- Abstract
- IgG4 Produkte
- Substanzklasse
- Antibody
- Hintergrund
- This recombinant Human IgG4 antibody reacts to the heavy chain of hIgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.
- Gen-ID
- 3503
- UniProt
- P01861
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