Rekombinanter 5-Methylcytosine Antikörper

Produktnummer ABIN4953287

Produktdetails

Target: 5-Methylcytosine

Antikörpertyp: Recombinant Antibody

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Methylated DNA Immunoprecipitation (MeDIP), Dot Blot (DB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (IHC)

Produktmerkmale: This recombinant 5mC antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.

Aufreinigung: Protein A purified from animal origin-free supernatant

Immunogen: BSA-conjugated 5-methylcytosine was used as the immunogen for this recombinant 5mC antibody.

Klon: RM231

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the recombinant 5mC antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot Blot: 0.5-2 μg/mL,ELISA: 0.1-1 μg/mL,Immunocytochemistry: 0.5-2 μg/mL,MeDIP: 0.2 μg/mL- 2 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the recombinant 5mC antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Antigendetails

Target: 5-Methylcytosine

Andere Bezeichnung: 5-Methylcytosine (5mC)

Substanzklasse: Chemical

Hintergrund: This recombinant 5mC antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.