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TNFRSF8 Antikörper (FITC)

TNFRSF8 Reaktivität: Human FACS Wirt: Maus Monoclonal Ber-H2 FITC
Produktnummer ABIN492586
  • Target Alle TNFRSF8 Antikörper anzeigen
    TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))
    Reaktivität
    • 139
    • 31
    • 18
    • 16
    Human
    Wirt
    • 90
    • 64
    • 14
    • 4
    • 1
    Maus
    Klonalität
    • 100
    • 71
    • 2
    Monoklonal
    Konjugat
    • 73
    • 14
    • 13
    • 12
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Dieser TNFRSF8 Antikörper ist konjugiert mit FITC
    Applikation
    • 105
    • 61
    • 60
    • 47
    • 38
    • 27
    • 26
    • 25
    • 13
    • 8
    • 6
    • 6
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Spezifität
    This antibody reacts with CD30 on Flow Cytometry.
    Kreuzreaktivität (Details)
    Species reactivity (tested):Human.
    Produktmerkmale
    Synonyms: TNFRSF8, D1S166E, CD30L receptor, KI-1 antigen, Tumor necrosis factor receptorsuperfamily member 8, Lymphocyte activation antigen CD30
    Aufreinigung
    Protein-A Agarose Chromatography of hybridoma supernatant.
    Immunogen
    Co cell line cellsRemarks: Hybridoma was established by fusion of mouse myeloma cell with Balb/c mousesplenocyte
    Klon
    Ber-H2
    Isotyp
    IgG1
    Top Product
    Discover our top product TNFRSF8 Primärantikörper
  • Applikationshinweise
    Flow Cytometry: 5-10 μg/mL (final concentration). Detailed procedure is provided in Protocols.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protokoll
    Flow cytometric analysis for floating cellsWe usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with washing buffer (5x10e6 cells/mL). 3) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute atRT (20~25°C). Remove supernatant by careful aspiration. 4) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0. 1% NaN3or 20 µL of Clear Back (human Fc receptor blocking reagent) to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 5) Add 20 µL of the FITC labeled CD30 monoclonal antibody (Ber-H2) (5-10 µg/mL) dilutedwith the washing buffer. Mix well and incubate for 30 minutes at RT (20~25°C). 6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at RT. Remove supernatant by careful aspiration. 7) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Positive Control: CCRF-CEMFlow cytometric analysis for whole blood cellsWe usually use Falcon tubes or equivalents as reaction tubes for all steps described below. 1) Add 20 µL of the FITC labeled CD163 monoclonal antibody (Ber-Mac3) (5-10 µg/mL)diluted with the washing buffer [PBS containing 2% fetal calf serum(FCS) and 0. 1% NaN3] into each tube. 2) Add 50 µL of whole blood into each tube. Mix well, and incubate for 30 minutes at RT(20~25°C). 3) Lyse with OptiLyse C (for analysis on Beckman Coulter instruments) or OptiLyse B (foranalysis on BD instruments), using the procedure recommended in the respective packageinserts. 4) Add 1mL of H2O to each tube and incubate for 10 minutes at RT (20~25°). 5) Centrifuge at 500 x g for 1 minute at RT (20~25°C). Remove supernatant by carefulaspiration. 6) Add 1 mL of washing buffer followed by centrifugation at 500 x g for 1 minute at RT(20~25°C). Remove supernatant by careful aspiration. 7) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    50 µg/mL
    Buffer
    PBS containing 5 % BSA and 0.09 % Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C.
    Shelf life: one year from despatch.
    Haltbarkeit
    12 months
  • Target
    TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))
    Andere Bezeichnung
    CD30 (TNFRSF8 Produkte)
    Synonyme
    CD30 antikoerper, D1S166E antikoerper, Ki-1 antikoerper, Cd30 antikoerper, Ki antikoerper, TNFRSF8 antikoerper, TNF receptor superfamily member 8 antikoerper, tumor necrosis factor receptor superfamily, member 8 antikoerper, TNFRSF8 antikoerper, Tnfrsf8 antikoerper
    Hintergrund
    CD30, also known as Ki-1, TNFRSF8, or Be-H2, is a 120 kDa glycoprotein expressed on the surface of mitogen-activated B-cells and T-cells but not on resting lymphocytes or monocytes. CD30 is also a marker for Hodgkin and Sternberg-Reed cells of Hodgkin's lymphomas and related hematologic malignancies. Soluble forms of CD30 have been found in the serum of patients with adult T-cell leukemia or other CD30+ lymphomas. The CD30 ligand, CD153, is a type II transmembrane glycoprotein that enhances proliferation of activated T-cells and induces apoptosis in CD30+ lymphoma-derived cell lines.Synonyms: CD30L receptor, D1S166E, KI-1 antigen, Lymphocyte activation antigen CD30, TNFRSF8, Tumor necrosis factor receptor superfamily member 8
    Gen-ID
    943
    UniProt
    P28908
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