KDELR1 Antikörper (C-Term)

Produktnummer ABIN487508

Produktdetails anti-KDELR1 Antikörper

Target: KDELR1 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1))

Bindungsspezifität: C-Term

Reaktivität: Drosophila melanogaster, Pflanzen, Saccharomyces cerevisiae

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KDELR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Spezifität: Detects ~78 kDa protein. The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in Yeast, Plant, nematode and other ER proteins. It specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).

Kreuzreaktivität (Details): Species reactivity (tested):Drosophila, Yeast, Saccharomyce, Plants (barnyard grass, beet, cotton, mung bean, sorghum and wheat).

Produktmerkmale: Synonyms: KDEL endoplasmic reticulum protein retention receptor 1, ERD2.1, ER marker, ER lumenprotein retaining receptor 1, Putative MAPK-activating protein PM23

Aufreinigung: Protein G Chromatography.

Immunogen: A synthetic HDEL peptide corresponding to the C-terminus of Yeast Bip.

Klon: 2-00E-007

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot: 1/1000-1/2000.1 μg/mL of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 μg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG -HRP asthe secondary antibody. Immunofluorescence: 1/50-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol with 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu KDELR1

Target: KDELR1 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1))

Andere Bezeichnung: KDEL Receptor 1 / KDELR1 (KDELR1 Produkte)

Synonyme: ERD2 antikoerper, ERD2.1 antikoerper, HDEL antikoerper, PM23 antikoerper, 8030486F04Rik antikoerper, AW215843 antikoerper, Erd2.1 antikoerper, KDEL endoplasmic reticulum protein retention receptor 1 antikoerper, KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1 antikoerper, KDELR1 antikoerper, Kdelr1 antikoerper

Hintergrund: HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3).Synonyms: ER lumen protein retaining receptor 1, ER marker, ERD2.1, KDEL endoplasmic reticulum protein retention receptor 1, Putative MAPK-activating protein PM23

Gen-ID: 10945

UniProt: P24390

Pathways: Maintenance of Protein Location