CCL2 Antikörper
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- Target Alle CCL2 Antikörper anzeigen
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
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Reaktivität
- Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser CCL2 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Spezifität
- Rat MCP-1
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Rat MCP-1.
Type of Immunogen: Cell extract - Top Product
- Discover our top product CCL2 Primärantikörper
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- Applikationshinweise
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Approved: ELISA (0.5 - 2 μg/mL), Neut (4 - 6 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained colchicine injected rat brain (including the caudate putamen) tissue. Frozen samples fixed in 4 % PFA. The primary antibody was incubated at 1 μg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat MCP-1 (MCAF) (100 ng/mL), a concentration of 4-6 μg/mL of this antibody is required. ELISA: To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat MCP-1(MCAF) (LS-C104762) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MCP-1(MCAF). Western Blot: To detect Rat MCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1 (MCAF) is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Kommentare
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Target Species of Antibody: Rat
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Sterile water
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handhabung
- Avoid freeze/thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
- Andere Bezeichnung
- CCL2 / MCP-1 (CCL2 Produkte)
- Synonyme
- GDCF-2 antikoerper, HC11 antikoerper, HSMCR30 antikoerper, MCAF antikoerper, MCP-1 antikoerper, MCP1 antikoerper, SCYA2 antikoerper, SMC-CF antikoerper, AI323594 antikoerper, JE antikoerper, Scya2 antikoerper, Sigje antikoerper, MCP-1A antikoerper, MCP1A antikoerper, C-C motif chemokine ligand 2 antikoerper, chemokine (C-C motif) ligand 2 antikoerper, C-C motif chemokine 2 antikoerper, CCL2 antikoerper, Ccl2 antikoerper, LOC101120093 antikoerper
- Hintergrund
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Name/Gene ID: CCL2
Subfamily: Intercrine beta
Family: Intercrine
Synonyms: CCL2, C-C motif chemokine 2, Chemokine (C-C motif) ligand 2, GDCF-2, HC11, HSMCR30, MCP1, SCYA2, Small-inducible cytokine A2, Monocyte secretory protein JE, MCAF, MCP-1, Monocyte chemotactic protein 1, SMC-CF - Gen-ID
- 6347
- UniProt
- P13500
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylierungen bei SARS-CoV-2 Infektion
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